Gamma-sterilization-induced radicals in biodegradable drug delivery systems

Karsten Mäder; Abraham Domb; Harold M. Swartz

doi:10.1016/S0969-8043(96)00236-9

Gamma-sterilization-induced radicals in biodegradable drug delivery systems

Karsten Mäder, Abraham Domb, Harold M. Swartz

Research output: Contribution to journal › Conference article › peer-review

35 Scopus citations

Abstract

Electron paramagnetic resonance (EPR) spectroscopy (1.2 and 9.25 GHz, 25°C) was used to characterize free radicals in gamma-ray sterilized biodegradable polymers of the type which are in clinical use. Free radicals were detected in all irradiated polymer samples. the temperature of irradiation (25°C vs dry ice temperature) had only a minor influence on the yield of radicals and the shape of the EPR spectra. In contrast, the composition of the polymers and the drugs incorporated in them did strongly influence the amount of radiation-induced free radicals and their reactivity. In general, polymers with high melting points and crystallinity had the highest yields of radicals observable at room temperature. We were able to use the free radicals induced by the usual sterilization procedures to follow the penetration of water and the degradation of the polymers in vitro and in vivo. The ability of in vivo EPR to follow drug delivery noninvasively and continuously in vivo, using the free radicals induced in the usual sterilization process indicates that this approach could be applied immediately for the characterization of these drug delivery systems in experimental animals and in the near future should be able to be used in human subjects.

Original language	English
Pages (from-to)	1669-1674
Number of pages	6
Journal	Applied Radiation and Isotopes
Volume	47
Issue number	11-12
DOIs	https://doi.org/10.1016/S0969-8043(96)00236-9
State	Published - Nov 1996
Externally published	Yes
Event	Proceedings of the 1995 4th International Symposium on ESR Dosimetry and Applications - Munich, Ger Duration: 15 May 1995 → 19 May 1995

Bibliographical note

Funding Information:
Acknowledgements--This researcuhs edt hef acilitieso f the IERC at Dartmouth, supportedb y NIH Grant P41RR01811K;a rstenM /iderg ratefullya cknowledgehsis support by DeutscherA kademischerA ustauschdienst (DAAD), Germany.

Funding

Acknowledgements--This researcuhs edt hef acilitieso f the IERC at Dartmouth, supportedb y NIH Grant P41RR01811K;a rstenM /iderg ratefullya cknowledgehsis support by DeutscherA kademischerA ustauschdienst (DAAD), Germany.

Funders	Funder number
DeutscherA kademischerA ustauschdienst
IERC
National Institutes of Health
National Center for Research Resources	P41RR001811

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10.1016/S0969-8043(96)00236-9

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title = "Gamma-sterilization-induced radicals in biodegradable drug delivery systems",

abstract = "Electron paramagnetic resonance (EPR) spectroscopy (1.2 and 9.25 GHz, 25°C) was used to characterize free radicals in gamma-ray sterilized biodegradable polymers of the type which are in clinical use. Free radicals were detected in all irradiated polymer samples. the temperature of irradiation (25°C vs dry ice temperature) had only a minor influence on the yield of radicals and the shape of the EPR spectra. In contrast, the composition of the polymers and the drugs incorporated in them did strongly influence the amount of radiation-induced free radicals and their reactivity. In general, polymers with high melting points and crystallinity had the highest yields of radicals observable at room temperature. We were able to use the free radicals induced by the usual sterilization procedures to follow the penetration of water and the degradation of the polymers in vitro and in vivo. The ability of in vivo EPR to follow drug delivery noninvasively and continuously in vivo, using the free radicals induced in the usual sterilization process indicates that this approach could be applied immediately for the characterization of these drug delivery systems in experimental animals and in the near future should be able to be used in human subjects.",

author = "Karsten M{\"a}der and Abraham Domb and Swartz, {Harold M.}",

note = "Funding Information: Acknowledgements--This researcuhs edt hef acilitieso f the IERC at Dartmouth, supportedb y NIH Grant P41RR01811K;a rstenM /iderg ratefullya cknowledgehsis support by DeutscherA kademischerA ustauschdienst (DAAD), Germany.; Proceedings of the 1995 4th International Symposium on ESR Dosimetry and Applications ; Conference date: 15-05-1995 Through 19-05-1995",

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PY - 1996/11

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N2 - Electron paramagnetic resonance (EPR) spectroscopy (1.2 and 9.25 GHz, 25°C) was used to characterize free radicals in gamma-ray sterilized biodegradable polymers of the type which are in clinical use. Free radicals were detected in all irradiated polymer samples. the temperature of irradiation (25°C vs dry ice temperature) had only a minor influence on the yield of radicals and the shape of the EPR spectra. In contrast, the composition of the polymers and the drugs incorporated in them did strongly influence the amount of radiation-induced free radicals and their reactivity. In general, polymers with high melting points and crystallinity had the highest yields of radicals observable at room temperature. We were able to use the free radicals induced by the usual sterilization procedures to follow the penetration of water and the degradation of the polymers in vitro and in vivo. The ability of in vivo EPR to follow drug delivery noninvasively and continuously in vivo, using the free radicals induced in the usual sterilization process indicates that this approach could be applied immediately for the characterization of these drug delivery systems in experimental animals and in the near future should be able to be used in human subjects.

AB - Electron paramagnetic resonance (EPR) spectroscopy (1.2 and 9.25 GHz, 25°C) was used to characterize free radicals in gamma-ray sterilized biodegradable polymers of the type which are in clinical use. Free radicals were detected in all irradiated polymer samples. the temperature of irradiation (25°C vs dry ice temperature) had only a minor influence on the yield of radicals and the shape of the EPR spectra. In contrast, the composition of the polymers and the drugs incorporated in them did strongly influence the amount of radiation-induced free radicals and their reactivity. In general, polymers with high melting points and crystallinity had the highest yields of radicals observable at room temperature. We were able to use the free radicals induced by the usual sterilization procedures to follow the penetration of water and the degradation of the polymers in vitro and in vivo. The ability of in vivo EPR to follow drug delivery noninvasively and continuously in vivo, using the free radicals induced in the usual sterilization process indicates that this approach could be applied immediately for the characterization of these drug delivery systems in experimental animals and in the near future should be able to be used in human subjects.

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Gamma-sterilization-induced radicals in biodegradable drug delivery systems

Abstract

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