TY - JOUR
T1 - Further purification and characterization of a thermophilic rennet
AU - Laxer, Shoshana
AU - Pnsky, Alex
AU - Bartoov, Benjamin
PY - 1981/11
Y1 - 1981/11
N2 - Further work on a thermophilic rennet synthesized by a thermophilic actinomycete is reported. It was produced by growth in fermentor of 50‐or‐200‐L volume and was purified by membrane filtration of a cell‐free supernatant and then molecular filtration and ion exchange chromatography. Its stability under various conditions was determined: The enzyme is a true rennet requiring calcium ions for activity. Experiment shows that it resembles neither pepsin, trypsin, nor chymotrypsin. It is freeze‐labile with a molecular weight of 9700 from amino acid composition. When skim milk powder was added to the growth medium, a rennet with different properties was obtained.
AB - Further work on a thermophilic rennet synthesized by a thermophilic actinomycete is reported. It was produced by growth in fermentor of 50‐or‐200‐L volume and was purified by membrane filtration of a cell‐free supernatant and then molecular filtration and ion exchange chromatography. Its stability under various conditions was determined: The enzyme is a true rennet requiring calcium ions for activity. Experiment shows that it resembles neither pepsin, trypsin, nor chymotrypsin. It is freeze‐labile with a molecular weight of 9700 from amino acid composition. When skim milk powder was added to the growth medium, a rennet with different properties was obtained.
UR - http://www.scopus.com/inward/record.url?scp=84941950619&partnerID=8YFLogxK
U2 - 10.1002/bit.260231108
DO - 10.1002/bit.260231108
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AN - SCOPUS:84941950619
SN - 0006-3592
VL - 23
SP - 2483
EP - 2492
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
IS - 11
ER -