Functionalised, photostable, fluorescent polystyrene nanoparticles of narrow size-distribution

Michal Pellach, Jenny Goldshtein, Ofra Ziv-Polat, Shlomo Margel

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Fluorescent nanoparticles continue to be of wide interest, as they have many advantages over single fluorescent molecules for biological imaging and sensing applications, such as increased fluorescence intensity and reduced photobleaching. In the following work, styrene was copolymerised with a newly synthesised, fluorescein-based, vinylic crosslinking monomer, by emulsion polymerisation, to create a series of different sized fluorescent nanoparticles (35-100 nm), each of narrow size-distribution. The particles were found to be highly fluorescent and with lower photobleaching compared to fluorescein isothiocyanate (FITC), offering an attractive alternative. The fluorescence excitation and emission spectra were recorded, being similar to fluorescein, but with interesting variation in the excitation spectra. The particles also have a wide range of potential uses, such as examining particle uptake activity of a macrophage cell line, also demonstrated. The nanoparticles were coated with albumin to provide functionality for potential conjugation to biological targeting agents.

Original languageEnglish
Pages (from-to)60-67
Number of pages8
JournalJournal of Photochemistry and Photobiology A: Chemistry
Issue number1
StatePublished - 15 Jan 2012


  • Emulsion polymerisation
  • Fluorescein
  • Fluorescent nanoparticles
  • Polystyrene nanoparticles


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