Functional photoacoustic microscopy of pH

Muhammad Rameez Chatni, Junjie Yao, Amos Danielli, Christopher P. Favazza, Konstantin I. Maslov, Lihong V. Wang

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


pH is a tightly regulated indicator of metabolic activity. In mammalian systems, an imbalance of pH regulation may result from or result in serious illness. In this paper, we report photoacoustic microscopy (PAM) of a commercially available pH-sensitive fluorescent dye (SNARF-5F carboxylic acid) in tissue phantoms. We demonstrated that PAM is capable of pH imaging in absolute values at tissue depths of up to 2.0 mm, greater than possible with other forms of optical microscopy.

Original languageEnglish
Article number100503
JournalJournal of Biomedical Optics
Issue number10
StatePublished - Oct 2011
Externally publishedYes

Bibliographical note

Funding Information:
The authors thank James Ballard and Sandra Matteucci for help in manuscript edition. This work was supported in part by National Institutes of Health Grant Nos. U54CA136398 and R01 EB000712.


  • SNARF-5F carboxylic acid
  • pH imaging
  • photoacoustic microscopy


Dive into the research topics of 'Functional photoacoustic microscopy of pH'. Together they form a unique fingerprint.

Cite this