Functional photoacoustic microscopy of pH

M. Rameez Chatni, Junjie Yao, Amos Danielli, Christopher P. Favazza, Konstantin I. Maslov, Lihong V. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


pH is a tightly regulated indicator of metabolic activity. In mammalian systems, imbalance of pH regulation may result from or result in serious illness. Even though the regulation system of pH is very robust, tissue pH can be altered in many diseases such as cancer, osteoporosis and diabetes mellitus. Traditional high-resolution optical imaging techniques, such as confocal microscopy, routinely image pH in cells and tissues using pH sensitive fluorescent dyes, which change their fluorescence properties with the surrounding pH. Since strong optical scattering in biological tissue blurs images at greater depths, high-resolution pH imaging is limited to penetration depths of 1mm. Here, we report photoacoustic microscopy (PAM) of commercially available pH-sensitive fluorescent dye in tissue phantoms. Using both opticalresolution photoacoustic microscopy (OR-PAM), and acoustic resolution photoacoustic microscopy (AR-PAM), we explored the possibility of recovering the pH values in tissue phantoms. In this paper, we demonstrate that PAM was capable of recovering pH values up to a depth of 2 mm, greater than possible with other forms of optical microscopy.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2012
ISBN (Print)9780819488664
StatePublished - 23 Feb 2012
Externally publishedYes
EventPhotons Plus Ultrasound: Imaging and Sensing 2012 - San Francisco, CA, United States
Duration: 22 Jan 201224 Jan 2012

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferencePhotons Plus Ultrasound: Imaging and Sensing 2012
Country/TerritoryUnited States
CitySan Francisco, CA


  • Optical imaging
  • PH
  • Photoacoustic imaging
  • Photoacoustic sensing
  • Quantitative photoacoustics


Dive into the research topics of 'Functional photoacoustic microscopy of pH'. Together they form a unique fingerprint.

Cite this