Functional characterization of a neuropeptide receptor exogenously expressed in Aplysia neurons

Guo Zhang, Shi-Qi Guo, Si-Yuan Yin, Wang-Ding Yuan, Ping Chen, Ji-Il Kim, Hui-Ying Wang, Hai-Bo Zhou, Abraham J. Susswein, Bong-Kiun Kaang, Jian Jing

Research output: Working paper / PreprintPreprint


Neuropeptides act mostly on a class of G-protein coupled receptors, and play a fundamental role in the functions of neural circuits underlying behaviors. However, the functions of neuropeptide receptors are poorly understood. Here, we used the mollusc model system Aplysia and microinjected the exogenous neuropeptide receptor apATRPR (Aplysia Allatotropin-related peptide receptor) with an expression vector (pNEX3) into Aplysia neurons that did not express the receptor. Physiological experiments demonstrated that apATRPR could mediate the excitability increase activated by its ligand, apATRP (Aplysia Allatotropin-related peptide), in the Aplysia neurons that now express the receptor. This study provides the first definitive evidence for a physiological function of a neuropeptide receptor in molluscan animals.Competing Interest StatementThe authors have declared no competing interest.
Original languageEnglish
Number of pages31
StatePublished - 14 Feb 2022

Publication series

PublisherCold Spring Harbor Laboratory


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