Functional analysis of protein ubiquitination

Gal Levy-Cohen, Michael Blank

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Abstract Alterations in the protein ubiquitination can lead to the development of serious pathological conditions and diseases and, therefore, are under extensive investigation. Here we detail the revised/updated version of two approaches for analyzing the functional activities of the ubiquitin transferring system and target protein ubiquitination. These approaches permit the analysis of protein ubiquitination within the cellular environment as well as in a tube when the purified components are used. The updates introduced in the protocols allow both to increase the sensitivity of the assays and to reduce the false positives often experienced in the analyses.

Original languageEnglish
Article number12075
Pages (from-to)37-39
Number of pages3
JournalAnalytical Biochemistry
Volume484
Early online date8 May 2015
DOIs
StatePublished - 1 Sep 2015

Bibliographical note

Publisher Copyright:
© 2015 Elsevier Inc.

Funding

This work was supported by a Marie-Curie CIG Grant (612816) and an Emanuel Kirshner Foundation grant awarded to M.B.

FundersFunder number
Emanuel Kirshner Foundation
Marie Curie612816

    Keywords

    • E3 ligases
    • Protein ubiquitination
    • Ubiquitin
    • Ubiquitin transferring system

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