Abstract
Abstract Alterations in the protein ubiquitination can lead to the development of serious pathological conditions and diseases and, therefore, are under extensive investigation. Here we detail the revised/updated version of two approaches for analyzing the functional activities of the ubiquitin transferring system and target protein ubiquitination. These approaches permit the analysis of protein ubiquitination within the cellular environment as well as in a tube when the purified components are used. The updates introduced in the protocols allow both to increase the sensitivity of the assays and to reduce the false positives often experienced in the analyses.
Original language | English |
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Article number | 12075 |
Pages (from-to) | 37-39 |
Number of pages | 3 |
Journal | Analytical Biochemistry |
Volume | 484 |
Early online date | 8 May 2015 |
DOIs | |
State | Published - 1 Sep 2015 |
Bibliographical note
Publisher Copyright:© 2015 Elsevier Inc.
Funding
This work was supported by a Marie-Curie CIG Grant (612816) and an Emanuel Kirshner Foundation grant awarded to M.B.
Funders | Funder number |
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Emanuel Kirshner Foundation | |
Marie Curie | 612816 |
Keywords
- E3 ligases
- Protein ubiquitination
- Ubiquitin
- Ubiquitin transferring system