Functional analysis of protein ubiquitination

Gal Levy-Cohen, Michael Blank

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Abstract Alterations in the protein ubiquitination can lead to the development of serious pathological conditions and diseases and, therefore, are under extensive investigation. Here we detail the revised/updated version of two approaches for analyzing the functional activities of the ubiquitin transferring system and target protein ubiquitination. These approaches permit the analysis of protein ubiquitination within the cellular environment as well as in a tube when the purified components are used. The updates introduced in the protocols allow both to increase the sensitivity of the assays and to reduce the false positives often experienced in the analyses.

Original languageEnglish
Article number12075
Pages (from-to)37-39
Number of pages3
JournalAnalytical Biochemistry
Early online date8 May 2015
StatePublished - 16 Jun 2015

Bibliographical note

Publisher Copyright:
© 2015 Elsevier Inc.


  • E3 ligases
  • Protein ubiquitination
  • Ubiquitin
  • Ubiquitin transferring system


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