From "carpet" mechanism to de-novo designed diastereomeric cell-selective antimicrobial peptides

Yechiel Shai, Ziv Oren

Research output: Contribution to journalReview articlepeer-review

344 Scopus citations

Abstract

Living organisms of all types produce a large repertoire of gene-encoded, net positively charged, antimicrobial peptides as part of their innate immunity to microbial invasion. Despite significant variations in composition, length and secondary structure most antimicrobial peptides are active in micromolar concentrations, suggesting a common general mechanism for their mode of action. Many antimicrobial peptides bind bacterial phospholipid membranes up to a threshold concentration, followed by membrane permeation/disintegration (the "carpet" mechanism). Recent data suggest that the details of the permeation pathways may vary for different peptides and are assigned to different modes of action. Accumulating data reveal that the molecular basis for cell selectivity is the ability of peptides to specifically bind the negatively charged bacterial membrane, as well as their oligomeric state in solution and in the membrane. Based on the "carpet" mechanism and the role of the peptide oligomeric state, a novel group of diastereomeric (containing D- and L-amino acids) antimicrobial peptides were developed. These peptides may serve as promising templates for the future designs of antimicrobial peptides.

Original languageEnglish
Pages (from-to)1629-1641
Number of pages13
JournalPeptides
Volume22
Issue number10
DOIs
StatePublished - Oct 2001
Externally publishedYes

Keywords

  • Antibacterial peptides
  • Diastereomers
  • Innate immunity
  • Lysis
  • Lytic peptide

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