Forming nanoparticles of α-amylase and embedding them into solid surfaces

David Meridor, Aharon Gedanken

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

In the current work nanoparticles (NPs) of α-amylase were generated in an aqueous solution using high-intensity ultrasound, and were subsequently immobilized on polyethylene (PE) films, or polycarbonate (PC) plates, or on microscope glass slides. The α-amylase NPs coated on the solid surfaces have been characterized by ESEM, TEM, FTIR, XPS and AFM. The substrates immobilized with α-amylase were used for hydrolyzing soluble potato starch to maltose. The amount of enzyme introduced in the substrates, leaching properties, and the catalytic activity of the immobilized enzyme were compared. The catalytic activity of the amylase deposited on the three solid surfaces was compared to that of the same amount of free enzyme at different pHs and temperatures. α-Amylase coated on PE showed the best catalytic activity in all the examined parameters when compared to native amylase, especially at high temperatures. When immobilized on glass, α-amylase showed better activity than the native enzyme over all pH and temperature values studied. However, the immobilization on PC did not improve the enzyme activity at any pH and any temperature compared to the free amylase. The kinetic parameters, Km and Vmax were also calculated. The amylase coated PE showed the most favorable kinetic parameters (Km = 5 g L-1 and V max = 5E-07 mol mL-1 min-1). In contrast, the anchored enzyme-PC exhibited unfavorable kinetic parameters (Km = 16 g L-1, Vmax = 4.2E-07 mol mL-1 min -1). The corresponding values for amylase-glass were Km = 7 g L-1, Vmax = 1.8E-07 mol mL-1 min -1, relative to those obtained for the free enzyme (Km = 6.6 g L-1, Vmax = 3.3E-07 mol mL-1 min -1).

Original languageEnglish
Pages (from-to)43-48
Number of pages6
JournalJournal of Molecular Catalysis - B Enzymatic
Volume90
DOIs
StatePublished - Jun 2013

Keywords

  • Amylase nanoparticles
  • Enzyme activity
  • Immobilization

Fingerprint

Dive into the research topics of 'Forming nanoparticles of α-amylase and embedding them into solid surfaces'. Together they form a unique fingerprint.

Cite this