TY - JOUR
T1 - Fluorescent probes and bioconjugation chemistries for single-molecule fluorescence analysis of biomolecules
AU - Kapanidis, Achillefs N.
AU - Weiss, Shimon
PY - 2002/12/22
Y1 - 2002/12/22
N2 - A popular method for probing single molecules is single-molecule fluorescence spectroscopy (SMFS). SMFS has allowed striking visualizations of single biomolecules at work, including the rotation of the rotor subunit of the rotary stepper motor protein F1-ATPase, the sliding of kinesin on microtubules, the translocation of RNA polymerase (RNAP) molecules on double-stranded DNA, the formation of ribozyme intermediates, and the real-time infection of a cell by single virus particles. This paper describes the properties of SMFS-compatible fluorophores, list considerations for popular assays, provides examples of site-specific labeling strategies that enabled SMFS observations, and looks at fluorophores and labeling strategies that can answer future challenges.
AB - A popular method for probing single molecules is single-molecule fluorescence spectroscopy (SMFS). SMFS has allowed striking visualizations of single biomolecules at work, including the rotation of the rotor subunit of the rotary stepper motor protein F1-ATPase, the sliding of kinesin on microtubules, the translocation of RNA polymerase (RNAP) molecules on double-stranded DNA, the formation of ribozyme intermediates, and the real-time infection of a cell by single virus particles. This paper describes the properties of SMFS-compatible fluorophores, list considerations for popular assays, provides examples of site-specific labeling strategies that enabled SMFS observations, and looks at fluorophores and labeling strategies that can answer future challenges.
UR - http://www.scopus.com/inward/record.url?scp=0037461520&partnerID=8YFLogxK
U2 - 10.1063/1.1521158
DO - 10.1063/1.1521158
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AN - SCOPUS:0037461520
SN - 0021-9606
VL - 117
SP - 10953
EP - 10964
JO - Journal of Chemical Physics
JF - Journal of Chemical Physics
IS - 24
ER -