Fluorescent Copper Probe Inhibiting Aβ1-16-Copper(II)-Catalyzed Intracellular Reactive Oxygen Species Production

Gulshan R. Walke; Dnyanesh S. Ranade; Shefali N. Ramteke; Srikanth Rapole; Cristina Satriano; Enrico Rizzarelli; Gaetano A. Tomaselli; Giuseppe Trusso Sfrazzetto; Prasad P. Kulkarni

doi:10.1021/acs.inorgchem.6b02915

Fluorescent Copper Probe Inhibiting Aβ1-16-Copper(II)-Catalyzed Intracellular Reactive Oxygen Species Production

Gulshan R. Walke
, Dnyanesh S. Ranade
, Shefali N. Ramteke
, Srikanth Rapole
, Cristina Satriano
, Enrico Rizzarelli
, Gaetano A. Tomaselli
, Giuseppe Trusso Sfrazzetto
, Prasad P. Kulkarni

Agharkar Research Institute
National Centre for Cell Science
University of Catania
Consorzio Interuniversitario di Ricerca in Chimica Dei Metalli Nei Sistemi Biologici
National Research Council of Italy

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A variety of fluorescent probes are proposed to monitor the intracellular copper content. So far, none of the probes have been evaluated for their potential to inhibit copper-associated intracellular oxidative stress. Herein, we studied the ability of a fluorescent copper probe, OBEP-CS1, to inhibit intracellular oxidative stress associated with an amyloid β (Aβ) peptide-copper complex. The data showed that OBEP-CS1 completely inhibits the copper-catalyzed oxidation as well as decarboxylation/deamination of Aβ1-16. Moreover, the cell imaging experiments confirmed that OBEP-CS1 can inhibit Aβ-Cu^II-catalyzed reactive oxygen species production in SH-SY5Y cells. We also demonstrated that Aβ1-16 peptide can bind intracellular copper and thereby exert oxidative stress.

Original language	English
Pages (from-to)	3729-3732
Number of pages	4
Journal	Inorganic Chemistry
Volume	56
Issue number	7
DOIs	https://doi.org/10.1021/acs.inorgchem.6b02915
State	Published - 3 Apr 2017
Externally published	Yes

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© 2017 American Chemical Society.

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10.1021/acs.inorgchem.6b02915

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@article{b4edfd587de1497bab24e76c46661827,

title = "Fluorescent Copper Probe Inhibiting Aβ1-16-Copper(II)-Catalyzed Intracellular Reactive Oxygen Species Production",

abstract = "A variety of fluorescent probes are proposed to monitor the intracellular copper content. So far, none of the probes have been evaluated for their potential to inhibit copper-associated intracellular oxidative stress. Herein, we studied the ability of a fluorescent copper probe, OBEP-CS1, to inhibit intracellular oxidative stress associated with an amyloid β (Aβ) peptide-copper complex. The data showed that OBEP-CS1 completely inhibits the copper-catalyzed oxidation as well as decarboxylation/deamination of Aβ1-16. Moreover, the cell imaging experiments confirmed that OBEP-CS1 can inhibit Aβ-CuII-catalyzed reactive oxygen species production in SH-SY5Y cells. We also demonstrated that Aβ1-16 peptide can bind intracellular copper and thereby exert oxidative stress.",

author = "Walke, \{Gulshan R.\} and Ranade, \{Dnyanesh S.\} and Ramteke, \{Shefali N.\} and Srikanth Rapole and Cristina Satriano and Enrico Rizzarelli and Tomaselli, \{Gaetano A.\} and \{Trusso Sfrazzetto\}, Giuseppe and Kulkarni, \{Prasad P.\}",

note = "Publisher Copyright: {\textcopyright} 2017 American Chemical Society.",

year = "2017",

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doi = "10.1021/acs.inorgchem.6b02915",

language = "אנגלית",

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T1 - Fluorescent Copper Probe Inhibiting Aβ1-16-Copper(II)-Catalyzed Intracellular Reactive Oxygen Species Production

AU - Walke, Gulshan R.

AU - Ranade, Dnyanesh S.

AU - Ramteke, Shefali N.

AU - Rapole, Srikanth

AU - Satriano, Cristina

AU - Rizzarelli, Enrico

AU - Tomaselli, Gaetano A.

AU - Trusso Sfrazzetto, Giuseppe

AU - Kulkarni, Prasad P.

PY - 2017/4/3

Y1 - 2017/4/3

N2 - A variety of fluorescent probes are proposed to monitor the intracellular copper content. So far, none of the probes have been evaluated for their potential to inhibit copper-associated intracellular oxidative stress. Herein, we studied the ability of a fluorescent copper probe, OBEP-CS1, to inhibit intracellular oxidative stress associated with an amyloid β (Aβ) peptide-copper complex. The data showed that OBEP-CS1 completely inhibits the copper-catalyzed oxidation as well as decarboxylation/deamination of Aβ1-16. Moreover, the cell imaging experiments confirmed that OBEP-CS1 can inhibit Aβ-CuII-catalyzed reactive oxygen species production in SH-SY5Y cells. We also demonstrated that Aβ1-16 peptide can bind intracellular copper and thereby exert oxidative stress.

AB - A variety of fluorescent probes are proposed to monitor the intracellular copper content. So far, none of the probes have been evaluated for their potential to inhibit copper-associated intracellular oxidative stress. Herein, we studied the ability of a fluorescent copper probe, OBEP-CS1, to inhibit intracellular oxidative stress associated with an amyloid β (Aβ) peptide-copper complex. The data showed that OBEP-CS1 completely inhibits the copper-catalyzed oxidation as well as decarboxylation/deamination of Aβ1-16. Moreover, the cell imaging experiments confirmed that OBEP-CS1 can inhibit Aβ-CuII-catalyzed reactive oxygen species production in SH-SY5Y cells. We also demonstrated that Aβ1-16 peptide can bind intracellular copper and thereby exert oxidative stress.

UR - https://www.scopus.com/pages/publications/85016929802

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JF - Inorganic Chemistry

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ER -

Fluorescent Copper Probe Inhibiting Aβ1-16-Copper(II)-Catalyzed Intracellular Reactive Oxygen Species Production

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