Fluorescent Copper Probe Inhibiting Aβ1-16-Copper(II)-Catalyzed Intracellular Reactive Oxygen Species Production

Gulshan R. Walke; Dnyanesh S. Ranade; Shefali N. Ramteke; Srikanth Rapole; Cristina Satriano; Enrico Rizzarelli; Gaetano A. Tomaselli; Giuseppe Trusso Sfrazzetto; Prasad P. Kulkarni

doi:10.1021/acs.inorgchem.6b02915

Fluorescent Copper Probe Inhibiting Aβ1-16-Copper(II)-Catalyzed Intracellular Reactive Oxygen Species Production

Gulshan R. Walke, Dnyanesh S. Ranade, Shefali N. Ramteke, Srikanth Rapole, Cristina Satriano, Enrico Rizzarelli, Gaetano A. Tomaselli, Giuseppe Trusso Sfrazzetto, Prasad P. Kulkarni

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9 Scopus citations

Abstract

A variety of fluorescent probes are proposed to monitor the intracellular copper content. So far, none of the probes have been evaluated for their potential to inhibit copper-associated intracellular oxidative stress. Herein, we studied the ability of a fluorescent copper probe, OBEP-CS1, to inhibit intracellular oxidative stress associated with an amyloid β (Aβ) peptide-copper complex. The data showed that OBEP-CS1 completely inhibits the copper-catalyzed oxidation as well as decarboxylation/deamination of Aβ1-16. Moreover, the cell imaging experiments confirmed that OBEP-CS1 can inhibit Aβ-Cu^II-catalyzed reactive oxygen species production in SH-SY5Y cells. We also demonstrated that Aβ1-16 peptide can bind intracellular copper and thereby exert oxidative stress.

Original language	English
Pages (from-to)	3729-3732
Number of pages	4
Journal	Inorganic Chemistry
Volume	56
Issue number	7
DOIs	https://doi.org/10.1021/acs.inorgchem.6b02915
State	Published - 3 Apr 2017
Externally published	Yes

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© 2017 American Chemical Society.

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10.1021/acs.inorgchem.6b02915

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title = "Fluorescent Copper Probe Inhibiting Aβ1-16-Copper(II)-Catalyzed Intracellular Reactive Oxygen Species Production",

abstract = "A variety of fluorescent probes are proposed to monitor the intracellular copper content. So far, none of the probes have been evaluated for their potential to inhibit copper-associated intracellular oxidative stress. Herein, we studied the ability of a fluorescent copper probe, OBEP-CS1, to inhibit intracellular oxidative stress associated with an amyloid β (Aβ) peptide-copper complex. The data showed that OBEP-CS1 completely inhibits the copper-catalyzed oxidation as well as decarboxylation/deamination of Aβ1-16. Moreover, the cell imaging experiments confirmed that OBEP-CS1 can inhibit Aβ-CuII-catalyzed reactive oxygen species production in SH-SY5Y cells. We also demonstrated that Aβ1-16 peptide can bind intracellular copper and thereby exert oxidative stress.",

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doi = "10.1021/acs.inorgchem.6b02915",

language = "אנגלית",

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AU - Walke, Gulshan R.

AU - Ranade, Dnyanesh S.

AU - Ramteke, Shefali N.

AU - Rapole, Srikanth

AU - Satriano, Cristina

AU - Rizzarelli, Enrico

AU - Tomaselli, Gaetano A.

AU - Trusso Sfrazzetto, Giuseppe

AU - Kulkarni, Prasad P.

PY - 2017/4/3

Y1 - 2017/4/3

N2 - A variety of fluorescent probes are proposed to monitor the intracellular copper content. So far, none of the probes have been evaluated for their potential to inhibit copper-associated intracellular oxidative stress. Herein, we studied the ability of a fluorescent copper probe, OBEP-CS1, to inhibit intracellular oxidative stress associated with an amyloid β (Aβ) peptide-copper complex. The data showed that OBEP-CS1 completely inhibits the copper-catalyzed oxidation as well as decarboxylation/deamination of Aβ1-16. Moreover, the cell imaging experiments confirmed that OBEP-CS1 can inhibit Aβ-CuII-catalyzed reactive oxygen species production in SH-SY5Y cells. We also demonstrated that Aβ1-16 peptide can bind intracellular copper and thereby exert oxidative stress.

AB - A variety of fluorescent probes are proposed to monitor the intracellular copper content. So far, none of the probes have been evaluated for their potential to inhibit copper-associated intracellular oxidative stress. Herein, we studied the ability of a fluorescent copper probe, OBEP-CS1, to inhibit intracellular oxidative stress associated with an amyloid β (Aβ) peptide-copper complex. The data showed that OBEP-CS1 completely inhibits the copper-catalyzed oxidation as well as decarboxylation/deamination of Aβ1-16. Moreover, the cell imaging experiments confirmed that OBEP-CS1 can inhibit Aβ-CuII-catalyzed reactive oxygen species production in SH-SY5Y cells. We also demonstrated that Aβ1-16 peptide can bind intracellular copper and thereby exert oxidative stress.

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Fluorescent Copper Probe Inhibiting Aβ1-16-Copper(II)-Catalyzed Intracellular Reactive Oxygen Species Production

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