Abstract
The fluorescence spectroscopy of single molecule dynamics and its biological applications were studied. It provided tools for testing semi-classical and quantum mechanical theories of light-matter interaction and molecular interactions. Properties of a single fluorescent probe attached to a macromolecule such as very high sensitivity of the fluorophore to its immediate local environment, including the sensitivity to the presence of other fluorophores and quenchers near-by were studied. Its unique absorption and emission transition dipoles, which could be interrogated by polarized light, were also investigated for providing local structural information.
| Original language | English |
|---|---|
| Pages (from-to) | 179-185 |
| Number of pages | 7 |
| Journal | Proceedings of SPIE - The International Society for Optical Engineering |
| Volume | 3273 |
| DOIs | |
| State | Published - 1998 |
| Externally published | Yes |
| Event | Laser Techniques for Condensed-Phase and Biological Systems - San Jose, CA, United States Duration: 29 Jan 1998 → 31 Jan 1998 |
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