TY - JOUR
T1 - Fluorescence spectroscopy of single molecule dynamics and its biological applications
AU - Ha, Taekjip
AU - Ting, Alice
AU - Liang, Joy
AU - Glass, Jennifer
AU - Chemla, Daniel
AU - Schultz, Pete
AU - Weiss, Shimon
PY - 1998
Y1 - 1998
N2 - The fluorescence spectroscopy of single molecule dynamics and its biological applications were studied. It provided tools for testing semi-classical and quantum mechanical theories of light-matter interaction and molecular interactions. Properties of a single fluorescent probe attached to a macromolecule such as very high sensitivity of the fluorophore to its immediate local environment, including the sensitivity to the presence of other fluorophores and quenchers near-by were studied. Its unique absorption and emission transition dipoles, which could be interrogated by polarized light, were also investigated for providing local structural information.
AB - The fluorescence spectroscopy of single molecule dynamics and its biological applications were studied. It provided tools for testing semi-classical and quantum mechanical theories of light-matter interaction and molecular interactions. Properties of a single fluorescent probe attached to a macromolecule such as very high sensitivity of the fluorophore to its immediate local environment, including the sensitivity to the presence of other fluorophores and quenchers near-by were studied. Its unique absorption and emission transition dipoles, which could be interrogated by polarized light, were also investigated for providing local structural information.
UR - http://www.scopus.com/inward/record.url?scp=0032225895&partnerID=8YFLogxK
U2 - 10.1117/12.306136
DO - 10.1117/12.306136
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AN - SCOPUS:0032225895
SN - 0277-786X
VL - 3273
SP - 179
EP - 185
JO - Proceedings of SPIE - The International Society for Optical Engineering
JF - Proceedings of SPIE - The International Society for Optical Engineering
T2 - Laser Techniques for Condensed-Phase and Biological Systems
Y2 - 29 January 1998 through 31 January 1998
ER -