Fluorescence Polarization as a Functional Parameter in Monitoring Living Cells: Theory and Practice

Mordechai Deutsch; Reuven Tirosh; Menachem Kaufman; Naomi Zurgil; Arye Weinreb

doi:10.1023/A:1015355018449

Fluorescence Polarization as a Functional Parameter in Monitoring Living Cells: Theory and Practice

Mordechai Deutsch, Reuven Tirosh, Menachem Kaufman, Naomi Zurgil, Arye Weinreb

Department of Physics

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The use of fluorescence polarization as a functional parameter in monitoring cellular activation calls for the reliable and accurate measurement of the fluorescence intensity and polarization (FI and FP) of microscopic objects. The relevant experimental parameters that enter such measurements are thoroughly discussed. The possibility of executing FP measurements properly by flow-through systems is compared with that of static cytometry. Remarks on the effects of high-power excitation on markers and cells conclude the paper.

Original language	English
Pages (from-to)	25-44
Number of pages	20
Journal	Journal of Fluorescence
Volume	12
Issue number	1
DOIs	https://doi.org/10.1023/A:1015355018449
State	Published - Mar 2002

Keywords

Excitation power density
Flow cytometry
Fluorescence
Individual living cells
Photo biostimulation
Photo-damage
Polarization
Static cytometry

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abstract = "The use of fluorescence polarization as a functional parameter in monitoring cellular activation calls for the reliable and accurate measurement of the fluorescence intensity and polarization (FI and FP) of microscopic objects. The relevant experimental parameters that enter such measurements are thoroughly discussed. The possibility of executing FP measurements properly by flow-through systems is compared with that of static cytometry. Remarks on the effects of high-power excitation on markers and cells conclude the paper.",

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Fluorescence Polarization as a Functional Parameter in Monitoring Living Cells: Theory and Practice

Abstract

Keywords

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