@inproceedings{7a377f3a39d3452f8de57382d476ff7c,
title = "Fluorescence lifetime microscopy with a time- And space-resolved single-photon counting detector",
abstract = "We have recently developed a wide-field photon-counting detector (the H33D detector) having high-temporal and high-spatial resolutions and capable of recording up to 500,000 photons per sec. Its temporal performance has been previously characterized using solutions of fluorescent materials with different lifetimes, and its spatial resolution using sub-diffraction objects (beads and quantum dots). Here we show its application to fluorescence lifetime imaging of live cells and compare its performance to a scanning confocal TCSPC approach. With the expected improvements in photocathode sensitivity and increase in detector throughput, this technology appears as a promising alternative to the current lifetime imaging solutions.",
keywords = "FLIM, Fluorescence, Lifetime, Live cell, Microchannel plate, Photocathode, Position sensitive anode, Quantum dot, Single-molecule, Single-photon",
author = "X. Michalet and Siegmund, {O. H.W.} and Vallerga, {J. V.} and P. Jelinsky and Pinaud, {F. F.} and Millaud, {I. E.} and S. Weiss",
year = "2006",
doi = "10.1117/12.686429",
language = "אנגלית",
isbn = "0819464708",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
booktitle = "Advanced Photon Counting Techniques",
note = "Advanced Photon Counting Techniques ; Conference date: 01-10-2006 Through 03-10-2006",
}