Fluorescence lifetime microscopy with a time- And space-resolved single-photon counting detector

X. Michalet, O. H.W. Siegmund, J. V. Vallerga, P. Jelinsky, F. F. Pinaud, I. E. Millaud, S. Weiss

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

10 Scopus citations


We have recently developed a wide-field photon-counting detector (the H33D detector) having high-temporal and high-spatial resolutions and capable of recording up to 500,000 photons per sec. Its temporal performance has been previously characterized using solutions of fluorescent materials with different lifetimes, and its spatial resolution using sub-diffraction objects (beads and quantum dots). Here we show its application to fluorescence lifetime imaging of live cells and compare its performance to a scanning confocal TCSPC approach. With the expected improvements in photocathode sensitivity and increase in detector throughput, this technology appears as a promising alternative to the current lifetime imaging solutions.

Original languageEnglish
Title of host publicationAdvanced Photon Counting Techniques
StatePublished - 2006
Externally publishedYes
EventAdvanced Photon Counting Techniques - Boston, MA, United States
Duration: 1 Oct 20063 Oct 2006

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X


ConferenceAdvanced Photon Counting Techniques
Country/TerritoryUnited States
CityBoston, MA


  • FLIM
  • Fluorescence
  • Lifetime
  • Live cell
  • Microchannel plate
  • Photocathode
  • Position sensitive anode
  • Quantum dot
  • Single-molecule
  • Single-photon


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