TY - GEN
T1 - Fluorescence intensity fluctuations in single-molecule polarization sensitive measurements
AU - Turgeman, Lior
AU - Fixler, Dror
PY - 2012
Y1 - 2012
N2 - A general framework to include fluctuations in the single molecule fluorescence intensity (FI) signal due to random changes in molecule dipole orientation was introduced at Optics Express (21, 2007). By assuming continuous changes in dipole orientation described by Brownian rotational diffusion, this research derives the probability density function (PDF) equation of FI fluctuations. Solution of the proposed equation for several limiting cases and different correlation times yields the short time behavior of FI fluctuations. Monte Carlo simulations results, in accordance with those found in theory will be presented during our talk.
AB - A general framework to include fluctuations in the single molecule fluorescence intensity (FI) signal due to random changes in molecule dipole orientation was introduced at Optics Express (21, 2007). By assuming continuous changes in dipole orientation described by Brownian rotational diffusion, this research derives the probability density function (PDF) equation of FI fluctuations. Solution of the proposed equation for several limiting cases and different correlation times yields the short time behavior of FI fluctuations. Monte Carlo simulations results, in accordance with those found in theory will be presented during our talk.
KW - Brownian rotational diffusion
KW - Fluorescence intensity fluctuations
KW - Molecule dipole reorientation
KW - Monte-Carlo simulation
KW - Single molecule fluorescence intensity
UR - http://www.scopus.com/inward/record.url?scp=84857591382&partnerID=8YFLogxK
U2 - 10.1117/12.907270
DO - 10.1117/12.907270
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AN - SCOPUS:84857591382
SN - 9780819488718
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Single Molecule Spectroscopy and Superresolution Imaging V
T2 - Single Molecule Spectroscopy and Superresolution Imaging V
Y2 - 21 January 2012 through 22 January 2012
ER -