Fluorescence attenuated by a thick scattering medium: Theory, simulations and experiments

Yitzchak Weber, Hamootal Duadi, Pavitra Sokke Rudraiah, Inbar Yariv, Gilad Yahav, Dror Fixler, Rinat Ankri

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Fluorescence-based imaging has an enormous impact on our understanding of biological systems. However, in vivo fluorescence imaging is greatly influenced by tissue scattering. A better understanding of this dependence can improve the potential of noninvasive in vivo fluorescence imaging. In this article, we present a diffusion model, based on an existing master–slave model, of isotropic point sources imbedded in a scattering slab, representing fluorophores within a tissue. The model was compared with Monte Carlo simulations and measurements of a fluorescent slide measured through tissue-like phantoms with different reduced scattering coefficients (0.5–2.5 mm−1) and thicknesses (0.5–5 mm). Results show a good correlation between our suggested theory, simulations and experiments; while the fluorescence intensity decays as the slab's scattering and thickness increase, the decay rate decreases as the reduced scattering coefficient increases in a counterintuitive manner, suggesting fewer fluorescence artifacts from deep within the tissue in highly scattering media.

Original languageEnglish
Article numbere202300045
JournalJournal of Biophotonics
Volume16
Issue number6
Early online date8 Mar 2023
DOIs
StatePublished - Jun 2023

Bibliographical note

Publisher Copyright:
© 2023 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.

Keywords

  • fluorescence imaging
  • fluorescence intensity
  • photon diffusion

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