Flow-cytometry Assessment of DNA content and Immunophenotyping of Immune-cells in Lymph-node-specimens as a Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas

David Azoulay, Tal Tapuchi, Ohad Ronen, Luiza Akria, Hector I. Cohen, Celia Surio, Svetlana Rodin Chepa, Elizabeth Eshel, Moran Zarfati, Galia Stemer, Netanel A. Horowitz

Research output: Contribution to journalArticlepeer-review

Abstract

Flow-cytometry (FC) is a powerful tool that can assist in lymphoma diagnosis in lymph node (LN) specimens. Although lymphoma diagnosis and classification are mainly based on tumor cell characteristics, surrounding cells are less employed in this process. We retrospectively investigated alterations in the ploidy status, proliferative cell fraction (PF) and the percentages of surrounding immune cells in 62 consecutive LN specimens with B-Cell Non-Hodgkin Lymphoma (B-NHL) that were submitted for FC evaluation between 2019–2022. Compared with indolent B-NHLs, aggressive B-NHLs show increased DNA aneuploidy and PF, increased monocytes, immature-granulocytes, mature granulocytes, CD8+ T-cells, Double-Negative-T-cells and Double-Positive-T-cells, and decreased total CD45+ cells, total lymphocytes, CD4+ T-cells and CD4/CD8 ratio. Receiver operating characteristic analysis determined PF > 6.8% and immature-granulocytes > 0.9% as optimal cutoffs with highest specificity and sensitivity in differentiating aggressive and indolent B-NHLs. These findings further strength the diagnostic value of DNA content analysis by FC and suggest the utilization of tumor surrounding immune cells in NHL diagnosis and classification.

Original languageEnglish
JournalAnnals of Hematology
Early online date23 May 2024
DOIs
StateE-pub ahead of print - 23 May 2024

Bibliographical note

Publisher Copyright:
© The Author(s) 2024.

Keywords

  • Cell
  • Cells, tumor
  • Cycle
  • Indexing, immune
  • Lymph
  • Lymphoma, DNA
  • Lymphoma, Indolent
  • Microenvironment
  • Node (LN), Aggrressive

Fingerprint

Dive into the research topics of 'Flow-cytometry Assessment of DNA content and Immunophenotyping of Immune-cells in Lymph-node-specimens as a Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas'. Together they form a unique fingerprint.

Cite this