Fast wide-field photothermal and quantitative phase cell imaging with optical lock-in detection

Will J. Eldridge, Amihai Meiri, Adi Sheinfeld, Matthew T. Rinehart, Adam Wax

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


We present a fast, wide-field holography system for detecting photothermally excited gold nanospheres with combined quantitative phase imaging. An interferometric photothermal optical lock-in approach (POLI) is shown to improve SNR for detecting nanoparticles (NPs) on multiple substrates, including a monolayer of NPs on a silanized coverslip, and NPs bound to live cells. Furthermore, the set up allowed for co-registered quantitative phase imaging (QPI) to be acquired in an off-axis holographic set-up. An SNR of 103 was obtained for NP-tagging of epidermal growth factor receptor (EGFR) in live cells with a 3 second acquisition, while an SNR of 47 was seen for 20 ms acquisition. An analysis of improvements in SNR due to averaging multiple frames is presented, which suggest that residual photothermal signal can be a limiting factor. The combination of techniques allows for high resolution imaging of cell structure via QPI with the ability to identify receptor expression via POLI.

Original languageEnglish
Pages (from-to)2517-2526
Number of pages10
JournalBiomedical Optics Express
Issue number8
StatePublished - 1 Aug 2014
Externally publishedYes


FundersFunder number
National Science FoundationMRI 1039562
National Science Foundation
Directorate for Engineering1039562


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