TY - JOUR
T1 - Fast wide-field photothermal and quantitative phase cell imaging with optical lock-in detection
AU - Eldridge, Will J.
AU - Meiri, Amihai
AU - Sheinfeld, Adi
AU - Rinehart, Matthew T.
AU - Wax, Adam
PY - 2014/8/1
Y1 - 2014/8/1
N2 - We present a fast, wide-field holography system for detecting photothermally excited gold nanospheres with combined quantitative phase imaging. An interferometric photothermal optical lock-in approach (POLI) is shown to improve SNR for detecting nanoparticles (NPs) on multiple substrates, including a monolayer of NPs on a silanized coverslip, and NPs bound to live cells. Furthermore, the set up allowed for co-registered quantitative phase imaging (QPI) to be acquired in an off-axis holographic set-up. An SNR of 103 was obtained for NP-tagging of epidermal growth factor receptor (EGFR) in live cells with a 3 second acquisition, while an SNR of 47 was seen for 20 ms acquisition. An analysis of improvements in SNR due to averaging multiple frames is presented, which suggest that residual photothermal signal can be a limiting factor. The combination of techniques allows for high resolution imaging of cell structure via QPI with the ability to identify receptor expression via POLI.
AB - We present a fast, wide-field holography system for detecting photothermally excited gold nanospheres with combined quantitative phase imaging. An interferometric photothermal optical lock-in approach (POLI) is shown to improve SNR for detecting nanoparticles (NPs) on multiple substrates, including a monolayer of NPs on a silanized coverslip, and NPs bound to live cells. Furthermore, the set up allowed for co-registered quantitative phase imaging (QPI) to be acquired in an off-axis holographic set-up. An SNR of 103 was obtained for NP-tagging of epidermal growth factor receptor (EGFR) in live cells with a 3 second acquisition, while an SNR of 47 was seen for 20 ms acquisition. An analysis of improvements in SNR due to averaging multiple frames is presented, which suggest that residual photothermal signal can be a limiting factor. The combination of techniques allows for high resolution imaging of cell structure via QPI with the ability to identify receptor expression via POLI.
UR - http://www.scopus.com/inward/record.url?scp=84904686140&partnerID=8YFLogxK
U2 - 10.1364/BOE.5.002517
DO - 10.1364/BOE.5.002517
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AN - SCOPUS:84904686140
SN - 2156-7085
VL - 5
SP - 2517
EP - 2526
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 8
ER -