Extremely sensitive dual imaging system in solid phantoms

Eran A. Barnoy; Dror Fixler; Rachela Popovtzer; Tsviya Nayhoz; Krishanu Ray

doi:10.1117/12.2207908

Extremely sensitive dual imaging system in solid phantoms

Eran A. Barnoy, Dror Fixler, Rachela Popovtzer, Tsviya Nayhoz, Krishanu Ray

University of Maryland, Baltimore

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Herein we describe promising results from the combination of fluorescent lifetime imaging microscopy (FLIM) and diffusion reflection (DR) medical imaging techniques. Three different geometries of gold nanoparticles (GNPs) were prepared: spheres of 20nm diameter, rods (GNRs) of aspect ratio (AR) 2.5, and GNRs of AR 3.3. Each GNP geometry was then conjugated using PEG linkers estimated to be 10nm in length to each of 3 different fluorescent dyes: Fluorescein, Rhodamine B, and Sulforhodamine B. DR provided deep-volume measurements (up to 1cm) from within solid, tissue-imitating phantoms, indicating GNR presence corresponding to the light used by recording light scattered from the GNPs with increasing distance to a photodetector. FLIM imaged solutions as well as phantom surfaces, recording both the fluorescence lifetimes as well as the fluorescence intensities. Fluorescence quenching was observed for Fluorescein, while metal-enhanced fluorescence (MEF) was observed in Rhodamine B and Sulforhodamine B - the dyes with an absorption peak at a slightly longer wavelength than the GNP plasmon resonance peak. Our system is highly sensitive due to the increased intensity provided by MEF, and also because of the inherent sensitivity of both FLIM and DR. Together, these two modalities and MEF can provide a lot of meaningful information for molecular and functional imaging of biological samples.

Original language	English
Title of host publication	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
Editors	Alexander N. Cartwright, Dan V. Nicolau
Publisher	SPIE
ISBN (Electronic)	9781628419559
DOIs	https://doi.org/10.1117/12.2207908
State	Published - 13 Feb 2016
Event	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII - San Francisco, United States Duration: 15 Feb 2016 → 17 Feb 2016

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	9721
ISSN (Print)	1605-7422

Conference

Conference	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
Country/Territory	United States
City	San Francisco
Period	15/02/16 → 17/02/16

Bibliographical note

Publisher Copyright:
© 2016 SPIE.

Funding

The work described in this manuscript was supported in part by the U.S. National Institutes of Health (Nos. AI087968 and NIGMS R01GM117836 (K.R.)).

Funders	Funder number
National Institutes of Health	AI087968
National Institute of General Medical Sciences	R01GM117836

Keywords

Gold nanoparticles
biomolecular imaging
diffusion reflection
fluorescence lifetime imaging
gold nanorods
metal enhanced fluorescence
noninvasive detection
tissue-imitating phantoms

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10.1117/12.2207908

Cite this

Barnoy, E. A., Fixler, D., Popovtzer, R., Nayhoz, T., & Ray, K. (2016). Extremely sensitive dual imaging system in solid phantoms. In A. N. Cartwright, & D. V. Nicolau (Eds.), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII Article 972106 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9721). SPIE. https://doi.org/10.1117/12.2207908

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Barnoy, EA, Fixler, D , Popovtzer, R, Nayhoz, T & Ray, K 2016, Extremely sensitive dual imaging system in solid phantoms. in AN Cartwright & DV Nicolau (eds), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII., 972106, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9721, SPIE, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII, San Francisco, United States, 15/02/16. https://doi.org/10.1117/12.2207908

Extremely sensitive dual imaging system in solid phantoms. / Barnoy, Eran A.; Fixler, Dror ; Popovtzer, Rachela et al.
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII. ed. / Alexander N. Cartwright; Dan V. Nicolau. SPIE, 2016. 972106 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9721).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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Extremely sensitive dual imaging system in solid phantoms

Abstract

Publication series

Conference

Bibliographical note

Funding

Keywords

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