TY - JOUR
T1 - Extranuclear protection of chromosomal DNA from oxidative stress
AU - Vanderauwera, Sandy
AU - Suzuki, Nobuhiro
AU - Miller, Gad
AU - Van De Cotte, Brigitte
AU - Morsa, Stijn
AU - Ravanat, Jean Luc
AU - Hegie, Alicia
AU - Triantaphylidès, Christian
AU - Shulaev, Vladimir
AU - Van Montagu, Marc C.E.
AU - Van Breusegem, Frank
AU - Mittler, Ron
PY - 2011/1/25
Y1 - 2011/1/25
N2 - Eukaryotic organisms evolved under aerobic conditions subjecting nuclear DNA to damage provoked by reactive oxygen species (ROS). Although ROS are thought to be a major cause of DNA damage, little is known about the molecular mechanisms protecting nuclear DNA from oxidative stress. Here we show that protection of nuclear DNA in plants requires a coordinated function of ROS-scavenging pathways residing in the cytosol and peroxisomes, demonstrating that nuclear ROS scavengers such as peroxiredoxin and glutathione are insufficient to safeguard DNA integrity. Both catalase (CAT2) and cytosolic ascorbate peroxidase (APX1) play a key role in protecting the plant genome against photorespiratory-dependent H2O2-induced DNA damage. In apx1/cat2 double-mutant plants, a DNA damage response is activated, suppressing growth via a WEE1 kinase-dependent cell-cycle checkpoint. This response is correlated with enhanced tolerance to oxidative stress, DNA stress-causing agents, and inhibited programmed cell death.
AB - Eukaryotic organisms evolved under aerobic conditions subjecting nuclear DNA to damage provoked by reactive oxygen species (ROS). Although ROS are thought to be a major cause of DNA damage, little is known about the molecular mechanisms protecting nuclear DNA from oxidative stress. Here we show that protection of nuclear DNA in plants requires a coordinated function of ROS-scavenging pathways residing in the cytosol and peroxisomes, demonstrating that nuclear ROS scavengers such as peroxiredoxin and glutathione are insufficient to safeguard DNA integrity. Both catalase (CAT2) and cytosolic ascorbate peroxidase (APX1) play a key role in protecting the plant genome against photorespiratory-dependent H2O2-induced DNA damage. In apx1/cat2 double-mutant plants, a DNA damage response is activated, suppressing growth via a WEE1 kinase-dependent cell-cycle checkpoint. This response is correlated with enhanced tolerance to oxidative stress, DNA stress-causing agents, and inhibited programmed cell death.
KW - Arabidopsis
KW - Hydrogen peroxide
KW - Stress tolerance
UR - https://www.scopus.com/pages/publications/79952182060
U2 - 10.1073/pnas.1018359108
DO - 10.1073/pnas.1018359108
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C2 - 21220338
AN - SCOPUS:79952182060
SN - 0027-8424
VL - 108
SP - 1711
EP - 1716
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 4
ER -