Expression, purification and crystallization of a PCI domain from the COP9 signalosome subunit 7 (CSN7)

Moshe Dessau; Daniel A. Chamovitz; Joel A. Hirsch

doi:10.1107/S1744309106041959

Expression, purification and crystallization of a PCI domain from the COP9 signalosome subunit 7 (CSN7)

Moshe Dessau
, Daniel A. Chamovitz
, Joel A. Hirsch

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A core fragment of Arabidopsis thaliana COP9 signalosome (CSN) subunit 7 was expressed in Escherichia coli. The protein was purified to homogeneity and screened for crystallization. Crystallization conditions were refined using the sitting-drop vapour-diffusion method. Crystals were obtained using polyethylene glycol 8000 as a precipitant and have a thick rod-like morphology. Their crystallographic symmetry is orthorhombic, space group C222₁, with unit-cell parameters a = 57.2, b = 86.2, c = 72.6 Å and a diffraction limit of 2.06 Å.

Original language	English
Pages (from-to)	1138-1140
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	62
Issue number	11
DOIs	https://doi.org/10.1107/S1744309106041959
State	Published - 1 Nov 2006
Externally published	Yes

Keywords

COP9 signalosome
PCI and MPN motifs
Proteasome

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10.1107/S1744309106041959

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title = "Expression, purification and crystallization of a PCI domain from the COP9 signalosome subunit 7 (CSN7)",

abstract = "A core fragment of Arabidopsis thaliana COP9 signalosome (CSN) subunit 7 was expressed in Escherichia coli. The protein was purified to homogeneity and screened for crystallization. Crystallization conditions were refined using the sitting-drop vapour-diffusion method. Crystals were obtained using polyethylene glycol 8000 as a precipitant and have a thick rod-like morphology. Their crystallographic symmetry is orthorhombic, space group C2221, with unit-cell parameters a = 57.2, b = 86.2, c = 72.6 {\AA} and a diffraction limit of 2.06 {\AA}.",

keywords = "COP9 signalosome, PCI and MPN motifs, Proteasome",

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AU - Dessau, Moshe

AU - Chamovitz, Daniel A.

AU - Hirsch, Joel A.

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N2 - A core fragment of Arabidopsis thaliana COP9 signalosome (CSN) subunit 7 was expressed in Escherichia coli. The protein was purified to homogeneity and screened for crystallization. Crystallization conditions were refined using the sitting-drop vapour-diffusion method. Crystals were obtained using polyethylene glycol 8000 as a precipitant and have a thick rod-like morphology. Their crystallographic symmetry is orthorhombic, space group C2221, with unit-cell parameters a = 57.2, b = 86.2, c = 72.6 Å and a diffraction limit of 2.06 Å.

AB - A core fragment of Arabidopsis thaliana COP9 signalosome (CSN) subunit 7 was expressed in Escherichia coli. The protein was purified to homogeneity and screened for crystallization. Crystallization conditions were refined using the sitting-drop vapour-diffusion method. Crystals were obtained using polyethylene glycol 8000 as a precipitant and have a thick rod-like morphology. Their crystallographic symmetry is orthorhombic, space group C2221, with unit-cell parameters a = 57.2, b = 86.2, c = 72.6 Å and a diffraction limit of 2.06 Å.

KW - COP9 signalosome

KW - PCI and MPN motifs

KW - Proteasome

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JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

IS - 11

ER -

Expression, purification and crystallization of a PCI domain from the COP9 signalosome subunit 7 (CSN7)

Abstract

Keywords

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