Expression of the nopaline synthase gene in Escherichia coll

Yedidya Gafni, Mary Dell Chilton

Research output: Contribution to journalArticlepeer-review


The Agrobacterium tumor-inducing (Ti) plasmid pTiT37 encodes nopaline synthase (NOS) gene (nos) with eukaryotic promoter elements that is expressed in transformed plant cells but not in the bacterial host. We have fused the nos gene to the Escherichia coli trp promoter, and observed synthesis of NOS in E. coli. The nopaline produced by this enzyme is excreted into the culture medium. NOS is enzymatically active at 30 °C but not 37 °C, as based on nopaline production. NOS protein is produced at both temperatures, based on production in minicells.

Original languageEnglish
Pages (from-to)141-146
Number of pages6
Issue number2-3
StatePublished - 1985
Externally publishedYes


  • A. tumefaciens
  • BAL31 exonuclease
  • IAA induction
  • Recombinant DNA
  • minicells
  • plasmids
  • trp promoter
  • λ Charon vectors


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