Expression of Active Human Interferon-β in Transgenic Plants

Orit Edelbaum, Dana Stein, Neta Holland, Yedidiah Gafni, Orna Livneh, Daniela Novick, Menachem Rubinstein, Ilan Sela

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Tobacco plants were transformed with the human gene for interferon-β (IFN-β). Transformation was determined by the polymerase chain reaction (PCR), and expression was determined by Western blot analysis, by purifying the IFN from the transgenic plants, and by bioassays indicating its activity in human cells. Plants expressing IFN-β were self-pollinated. IFN-β-expressing progeny plants were selected and produced active IFN-β, indicating stable transformation.

Original languageEnglish
Pages (from-to)449-453
Number of pages5
JournalJournal of Interferon Research
Volume12
Issue number6
DOIs
StatePublished - Dec 1992
Externally publishedYes

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