Abstract
Pasteurella multocida serogroup B:2, a causative agent of haemorrhagic secpticaemia (HS) in cattle and buffalo especially in tropical regions of Asia and African countries, is known to possess a type IV fimbriae (pili) as one of the virulent factors. In the present study, ptfA gene encoding for type IV fimbrial subunit of P. multocida serogroup B:2 (strain p52), an Indian HS vaccine strain, has been cloned and over-expressed in recombinant Escherichia coli. The recombinant type IV fimbrial subunit protein (∼31. kDa) including N-terminus histidine tag was purified under denaturing condition and confirmed by western blotting. A homology model of HS causing P. multocida serogroup B:2 fimbrial subunit has also been discussed. The study indicated the potential possibilities to use the recombinant fimbrial protein in developing HS subunit vaccine along with suitable adjuvant.
| Original language | English |
|---|---|
| Pages (from-to) | 1128-1131 |
| Number of pages | 4 |
| Journal | Research in Veterinary Science |
| Volume | 93 |
| Issue number | 3 |
| DOIs | |
| State | Published - Dec 2012 |
| Externally published | Yes |
Funding
Authors are thankful to the Head, Division of Virology, IVRI-Mukteshwar; the Director, IVRI-Izatnagar; the Indian Council of Agricultural Research (ICAR), New Delhi; and Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India, New Delhi, for providing the necessary facilities to carry out the research on P. multocida and preparation of manuscript. Authors are also thankful to Division of Bacteriology and Mycology, IVRI-Izatnagar for providing P. multocida serogroup B:2 (strain p52).
| Funders | Funder number |
|---|---|
| IVRI-Mukteshwar | |
| Department of Biotechnology, Ministry of Science and Technology, India | |
| Indian Council of Agricultural Research | |
| Ministry of Science and Technology, Taiwan |
Keywords
- Expression
- Fimbrial subunit
- Pasteurella multocida
- PtfA
- Serogroup B:2
