Expression and purification of recombinant type IV fimbrial subunit protein of Pasteurella multocida serogroup B:2 in Escherichia coli

Sathish B. Shivachandra, Revanaiah Yogisharadhya, Anuj Ahuja, Veerakyathappa Bhanuprakash

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Pasteurella multocida serogroup B:2, a causative agent of haemorrhagic secpticaemia (HS) in cattle and buffalo especially in tropical regions of Asia and African countries, is known to possess a type IV fimbriae (pili) as one of the virulent factors. In the present study, ptfA gene encoding for type IV fimbrial subunit of P. multocida serogroup B:2 (strain p52), an Indian HS vaccine strain, has been cloned and over-expressed in recombinant Escherichia coli. The recombinant type IV fimbrial subunit protein (∼31. kDa) including N-terminus histidine tag was purified under denaturing condition and confirmed by western blotting. A homology model of HS causing P. multocida serogroup B:2 fimbrial subunit has also been discussed. The study indicated the potential possibilities to use the recombinant fimbrial protein in developing HS subunit vaccine along with suitable adjuvant.

Original languageEnglish
Pages (from-to)1128-1131
Number of pages4
JournalResearch in Veterinary Science
Volume93
Issue number3
DOIs
StatePublished - Dec 2012
Externally publishedYes

Keywords

  • Expression
  • Fimbrial subunit
  • Pasteurella multocida
  • PtfA
  • Serogroup B:2

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