Expression analysis using DNA microarrays demonstrates that E2F-1 up-regulates expression of DNA replication genes including replication protein A2

Y. Kalma; L. Marash; Y. Lamed; D. Ginsberg

doi:10.1038/sj.onc.1204230

Expression analysis using DNA microarrays demonstrates that E2F-1 up-regulates expression of DNA replication genes including replication protein A2

Y. Kalma, L. Marash, Y. Lamed, D. Ginsberg

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

The transcription factor E2F-1 plays a pivotal role in the regulation of G1/S transition in higher eukaryotes cell cycle. We used a cell line containing an inducible E2F-1 and oligonucleotide microarray analysis to identify novel E2F target genes. We show that E2F-1 up-regulates the expression of a number of genes coding for components of the DNA replication machinery. Among them is the gene coding for the 32 Kd subunit of replication protein A (RPA2). Replication protein A is the most abundant single strand DNA binding complex and it is essential for DNA replication. We demonstrate that RPA2 is a novel E2F target gene whose expression can be directly regulated by E2F-1 via E2F binding sites in its promoter. In addition, expression of Topoisomerase IIα and subunit IV of DNA polymerase α is also up-regulated upon E2F-1 induction. Taken together, these results provide novel links between components of the DNA replication machinery and the cell growth regulatory pathway involving the Rb tumor suppressor and E2F.

Original language	English
Pages (from-to)	1379-1387
Number of pages	9
Journal	Oncogene
Volume	20
Issue number	11
DOIs	https://doi.org/10.1038/sj.onc.1204230
State	Published - 15 Mar 2001
Externally published	Yes

Bibliographical note

Funding Information:
We thank Drs WG Kaelin Jr and PD Adams for cells lines and Dr D Givol for reagents and critical reading of the manuscript. We thank Dr Shirley Horn-Saban for excellent technical assistance with DNA microarray analysis. This work was supported in part by grants from the Israel cancer Association (ICA), Minerva Foundation (Germany), the Israel Cancer Research Fund (ICRF) and Yad Abraham Research Center for Diagnostics and Therapy. D Ginsburg is an incumbent of the Recanati Career Development chair of cancer research.

Funding

We thank Drs WG Kaelin Jr and PD Adams for cells lines and Dr D Givol for reagents and critical reading of the manuscript. We thank Dr Shirley Horn-Saban for excellent technical assistance with DNA microarray analysis. This work was supported in part by grants from the Israel cancer Association (ICA), Minerva Foundation (Germany), the Israel Cancer Research Fund (ICRF) and Yad Abraham Research Center for Diagnostics and Therapy. D Ginsburg is an incumbent of the Recanati Career Development chair of cancer research.

Funders	Funder number
Yad Abraham Research Center for Diagnostics and Therapy
Israel Cancer Research Fund
Minerva Foundation
Israel Cancer Association

Keywords

Cell cycle
DNA chips
E2F
RPA

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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abstract = "The transcription factor E2F-1 plays a pivotal role in the regulation of G1/S transition in higher eukaryotes cell cycle. We used a cell line containing an inducible E2F-1 and oligonucleotide microarray analysis to identify novel E2F target genes. We show that E2F-1 up-regulates the expression of a number of genes coding for components of the DNA replication machinery. Among them is the gene coding for the 32 Kd subunit of replication protein A (RPA2). Replication protein A is the most abundant single strand DNA binding complex and it is essential for DNA replication. We demonstrate that RPA2 is a novel E2F target gene whose expression can be directly regulated by E2F-1 via E2F binding sites in its promoter. In addition, expression of Topoisomerase IIα and subunit IV of DNA polymerase α is also up-regulated upon E2F-1 induction. Taken together, these results provide novel links between components of the DNA replication machinery and the cell growth regulatory pathway involving the Rb tumor suppressor and E2F.",

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Expression analysis using DNA microarrays demonstrates that E2F-1 up-regulates expression of DNA replication genes including replication protein A2

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

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