Exogenous expression of an allatotropin-related peptide receptor increased the membrane excitability in Aplysia neurons

Guo Zhang; Shi Qi Guo; Si Yuan Yin; Wang Ding Yuan; Ping Chen; Ji il Kim; Hui Ying Wang; Hai Bo Zhou; Abraham J. Susswein; Bong Kiun Kaang; Jian Jing

doi:10.1186/s13041-022-00929-4

Exogenous expression of an allatotropin-related peptide receptor increased the membrane excitability in Aplysia neurons

Guo Zhang, Shi Qi Guo, Si Yuan Yin, Wang Ding Yuan, Ping Chen, Ji il Kim, Hui Ying Wang, Hai Bo Zhou, Abraham J. Susswein, Bong Kiun Kaang, Jian Jing

The Mina and Everard Goodman Faculty of Life Sciences

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Neuropeptides act mostly on a class of G-protein coupled receptors, and play a fundamental role in the functions of neural circuits underlying behaviors. However, physiological functions of some neuropeptide receptors are poorly understood. Here, we used the molluscan model system Aplysia and microinjected the exogenous neuropeptide receptor apATRPR (Aplysia allatotropin-related peptide receptor) with an expression vector (pNEX3) into Aplysia neurons that did not express the receptor endogenously. Physiological experiments demonstrated that apATRPR could mediate the excitability increase induced by its ligand, apATRP (Aplysia allatotropin-related peptide), in the Aplysia neurons that now express the receptor. This study provides a definitive evidence for a physiological function of a neuropeptide receptor in molluscan animals.

Original language	English
Article number	42
Journal	Molecular Brain
Volume	15
Issue number	1
Early online date	9 May 2022
DOIs	https://doi.org/10.1186/s13041-022-00929-4
State	Published - 9 May 2022

Bibliographical note

Publisher Copyright:
© 2022, The Author(s).

Funding

This work was supported by the National Natural Science Foundation of China (Grants 62050071, 32171011, 32100816, 31861143036), Natural Science Foundation of Jiangsu Province (Grant BK20210183), the National Research Foundation of Korea (Grant NRF-2012R1A3A1050385) and Israel Science Foundation Grant 2396/18.

Funders	Funder number
National Natural Science Foundation of China	31861143036, 32100816, 62050071, 32171011
National Research Foundation of Korea	NRF-2012R1A3A1050385
Israel Science Foundation	2396/18
Natural Science Foundation of Jiangsu Province	BK20210183

Keywords

Aplysia
Electrophysiology
G-protein coupled receptors
Neuronal excitability
Neuropeptide
Plasmid microinjection

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10.1186/s13041-022-00929-4

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title = "Exogenous expression of an allatotropin-related peptide receptor increased the membrane excitability in Aplysia neurons",

abstract = "Neuropeptides act mostly on a class of G-protein coupled receptors, and play a fundamental role in the functions of neural circuits underlying behaviors. However, physiological functions of some neuropeptide receptors are poorly understood. Here, we used the molluscan model system Aplysia and microinjected the exogenous neuropeptide receptor apATRPR (Aplysia allatotropin-related peptide receptor) with an expression vector (pNEX3) into Aplysia neurons that did not express the receptor endogenously. Physiological experiments demonstrated that apATRPR could mediate the excitability increase induced by its ligand, apATRP (Aplysia allatotropin-related peptide), in the Aplysia neurons that now express the receptor. This study provides a definitive evidence for a physiological function of a neuropeptide receptor in molluscan animals.",

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doi = "10.1186/s13041-022-00929-4",

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AU - Zhang, Guo

AU - Guo, Shi Qi

AU - Yin, Si Yuan

AU - Yuan, Wang Ding

AU - Chen, Ping

AU - Kim, Ji il

AU - Wang, Hui Ying

AU - Zhou, Hai Bo

AU - Susswein, Abraham J.

AU - Kaang, Bong Kiun

AU - Jing, Jian

PY - 2022/5/9

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AB - Neuropeptides act mostly on a class of G-protein coupled receptors, and play a fundamental role in the functions of neural circuits underlying behaviors. However, physiological functions of some neuropeptide receptors are poorly understood. Here, we used the molluscan model system Aplysia and microinjected the exogenous neuropeptide receptor apATRPR (Aplysia allatotropin-related peptide receptor) with an expression vector (pNEX3) into Aplysia neurons that did not express the receptor endogenously. Physiological experiments demonstrated that apATRPR could mediate the excitability increase induced by its ligand, apATRP (Aplysia allatotropin-related peptide), in the Aplysia neurons that now express the receptor. This study provides a definitive evidence for a physiological function of a neuropeptide receptor in molluscan animals.

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KW - Electrophysiology

KW - G-protein coupled receptors

KW - Neuronal excitability

KW - Neuropeptide

KW - Plasmid microinjection

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Exogenous expression of an allatotropin-related peptide receptor increased the membrane excitability in Aplysia neurons

Abstract

Bibliographical note

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Keywords

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