Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics

Hadar Bootz-Maoz, Ariel Simon, Sara Del Mare-Roumani, Yifat Bennet, Einat Toister, Hadar Romano, Danping Zheng, Sivan Amidror, Eran Elinav, Nissan Yissachar

Research output: Contribution to journalArticlepeer-review


The intestinal epithelial barrier facilitates homeostatic host–microbiota interactions and immunological tolerance. However, mechanistic dissections of barrier dynamics following luminal stimulation pose a substantial challenge. Here, we describe an ex vivo intestinal permeability assay, X-IPA, for quantitative analysis of gut permeability dynamics at the whole-tissue level. We demonstrate that specific gut microbes and metabolites induce rapid, dose-dependent increases to gut permeability, thus providing a powerful approach for precise investigation of barrier functions.

Original languageEnglish
Article number44
Journalnpj Biofilms and Microbiomes
Issue number1
StatePublished - Dec 2023

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© 2023, The Author(s).


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