TY - JOUR
T1 - Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics
AU - Bootz-Maoz, Hadar
AU - Simon, Ariel
AU - Del Mare-Roumani, Sara
AU - Bennet, Yifat
AU - Toister, Einat
AU - Romano, Hadar
AU - Zheng, Danping
AU - Amidror, Sivan
AU - Elinav, Eran
AU - Yissachar, Nissan
N1 - Publisher Copyright:
© 2023, The Author(s).
PY - 2023/12
Y1 - 2023/12
N2 - The intestinal epithelial barrier facilitates homeostatic host–microbiota interactions and immunological tolerance. However, mechanistic dissections of barrier dynamics following luminal stimulation pose a substantial challenge. Here, we describe an ex vivo intestinal permeability assay, X-IPA, for quantitative analysis of gut permeability dynamics at the whole-tissue level. We demonstrate that specific gut microbes and metabolites induce rapid, dose-dependent increases to gut permeability, thus providing a powerful approach for precise investigation of barrier functions.
AB - The intestinal epithelial barrier facilitates homeostatic host–microbiota interactions and immunological tolerance. However, mechanistic dissections of barrier dynamics following luminal stimulation pose a substantial challenge. Here, we describe an ex vivo intestinal permeability assay, X-IPA, for quantitative analysis of gut permeability dynamics at the whole-tissue level. We demonstrate that specific gut microbes and metabolites induce rapid, dose-dependent increases to gut permeability, thus providing a powerful approach for precise investigation of barrier functions.
UR - http://www.scopus.com/inward/record.url?scp=85164000998&partnerID=8YFLogxK
U2 - 10.1038/s41522-023-00409-0
DO - 10.1038/s41522-023-00409-0
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C2 - 37400593
AN - SCOPUS:85164000998
SN - 2055-5008
VL - 9
JO - npj Biofilms and Microbiomes
JF - npj Biofilms and Microbiomes
IS - 1
M1 - 44
ER -