Abstract
Human kidney cells quickly degenerate in culture, precluding them as a source for cell therapy for chronic kidney disease (CKD). Herein, Harari-Steinberg et al. show that upon culturing as 3D nephrospheres, human kidney cells regain their epithelial phenotype and acquire in vivo tissue-forming and reparative properties in a CKD mouse model.
Original language | English |
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Pages (from-to) | 852-869.e4 |
Journal | Cell Reports |
Volume | 30 |
Issue number | 3 |
DOIs | |
State | Published - 21 Jan 2020 |
Bibliographical note
Publisher Copyright:© 2019 The Authors
Funding
We wish to thank the entire staff at the Pediatric Stem Cell Research Institute at the Sheba Medical Center for the fruitful discussions. We wish to thank Dr. Tali Bar-Lev from the Faculty of Engineering and Bar-Ilan Institute of Nanotechnology and Advanced Materials (BINA) at Bar-Ilan University for assistance with the bioinformatic experiments, Michael Namestnikov for assistance with generating the summarizing scheme, and the Israel National Center for Personalized Medicine (INCMP) for their assistance in sequencing and analysis. This study was supported by KidneyCure Bio and the Sheba Medical Center (B.D.) and the Israel Science Foundation (ICORE 1902/12 and grants 1634/13 and 2017/13), the Israel Ministry of Health (grant 3-10146), and the European Union (EU) Seventh Framework Programme (FP7) (Marie Curie International Reintegration grant 618592) (T.K.). Experiment Design, O.H.-S. D.O. and B.D.; Experiment Performance, D.O. Y.G. S.G. O.C.-Z. S.P.-C. Y.F. and Z.D.; Data Analysis and Manuscript Writing, O.H.-S. D.O. O.P. and B.D.; RNA Quality Checks for Sequencing, I.K. and T.K.; RNA-Seq Data Preprocessing and Analysis, O.H.S, O.C.-Z. I.K. N.B.H. E.B. and T.K. B.D is a co-founder, shareholder, and board member at KidneyCure. O.H.S is a shareholder at KidneyCure. We wish to thank the entire staff at the Pediatric Stem Cell Research Institute at the Sheba Medical Center for the fruitful discussions. We wish to thank Dr. Tali Bar-Lev from the Faculty of Engineering and Bar-Ilan Institute of Nanotechnology and Advanced Materials (BINA) at Bar-Ilan University for assistance with the bioinformatic experiments, Michael Namestnikov for assistance with generating the summarizing scheme, and the Israel National Center for Personalized Medicine (INCMP) for their assistance in sequencing and analysis. This study was supported by KidneyCure Bio and the Sheba Medical Center (B.D.) and the Israel Science Foundation (ICORE 1902/12 and grants 1634/13 and 2017/13 ), the Israel Ministry of Health (grant 3-10146 ), and the European Union ( EU ) Seventh Framework Programme ( FP7 ) (Marie Curie International Reintegration grant 618592 ) (T.K.).
Funders | Funder number |
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BINA | |
Faculty of Engineering and Bar-Ilan Institute of Nanotechnology and Advanced Materials | |
INCMP | |
Israel Ministry of Health | |
Israel National Center for Personalized Medicine | |
KidneyCure Bio | |
Marie Curie International Reintegration | |
Pediatric Stem Cell Research Institute at the Sheba Medical Center | |
Sheba Medical Center | |
Union ( EU ) Seventh Framework Programme | |
European Commission | |
Israel Science Foundation | 2017/13, ICORE 1902/12, 1634/13 |
Seventh Framework Programme | 618592 |
Ministry of Health, State of Israel | 3-10146 |
Keywords
- 3D culture
- 5/6 nephrectomy
- antifibrotic effect
- autologous treatment
- in vivo differentiation
- kidney regeneration
- kidney stem cells
- mouse CKD model
- nephrospheres
- renal epithelial differentiation