Ex Vivo Expanded 3D Human Kidney Spheres Engraft Long Term and Repair Chronic Renal Injury in Mice

Orit Harari-Steinberg, Dorit Omer, Yehudit Gnatek, Oren Pleniceanu, Sanja Goldberg, Osnat Cohen-Zontag, Sara Pri-Chen, Itamar Kanter, Nissim Ben Haim, Eli Becker, Roi Ankawa, Yaron Fuchs, Tomer Kalisky, Zohar Dotan, Benjamin Dekel

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Human kidney cells quickly degenerate in culture, precluding them as a source for cell therapy for chronic kidney disease (CKD). Herein, Harari-Steinberg et al. show that upon culturing as 3D nephrospheres, human kidney cells regain their epithelial phenotype and acquire in vivo tissue-forming and reparative properties in a CKD mouse model.

Original languageEnglish
Pages (from-to)852-869.e4
JournalCell Reports
Volume30
Issue number3
DOIs
StatePublished - 21 Jan 2020

Bibliographical note

Publisher Copyright:
© 2019 The Authors

Keywords

  • 3D culture
  • 5/6 nephrectomy
  • antifibrotic effect
  • autologous treatment
  • in vivo differentiation
  • kidney regeneration
  • kidney stem cells
  • mouse CKD model
  • nephrospheres
  • renal epithelial differentiation

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