Evidence for a functional role of acetylcholinesterase in cultured chick myotubes

S. R. Sampson, Tamar Babila, Marie Helene Disatnik, A. Shainberg, Esther Yoles

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1 Scopus citations

Abstract

This study was undertaken in order to assess the functional role of acetylcholinesterase (AChE) in cultures of chick skeletal muscle cells. Cultures of skeletal myotubes were prepared by mechanical dissociation of limb muscle removed from 11-day-old chick embryos and plating at a concentration of 0.8 × 106 cells/ml. Cultures incubated for 4-10 days were used for electrophysiological studies with intracellular microelectrodes. Individual myotubes differed with respect to the time course of repolarization following depolarization by acetylcholine (ACh), some cells repolarizing within 2-3 min and others only after 8-10 min. Physostigmine (10-8-10-6 M) prolonged or sometimes completely prevented repolarization following ACh-induced depolarization. These results demonstrate that hydrolysis of ACh by AChE in cultured chick skeletal myotubes plays an important role in the repolarization of these cells following ACh-induced depolarization.

Original languageEnglish
Pages (from-to)338-341
Number of pages4
JournalBrain Research
Volume289
Issue number1-2
DOIs
StatePublished - 19 Dec 1983

Bibliographical note

Funding Information:
This work was supporteidn part by fundsf romthe ResearchA uthorityB ar-IlanU niversityW. e wish to thank Ms. Asia Bek for preparatioonf musclec ul-tures.

Keywords

  • acetylcholine receptor
  • acetylcholinesterase
  • desensitization
  • muscle cultures

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