Evidence for a functional role of acetylcholinesterase in cultured chick myotubes

S. R. Sampson; Tamar Babila; Marie Helene Disatnik; A. Shainberg; Esther Yoles

doi:10.1016/0006-8993(83)90038-0

Evidence for a functional role of acetylcholinesterase in cultured chick myotubes

S. R. Sampson, Tamar Babila, Marie Helene Disatnik, A. Shainberg, Esther Yoles

The Mina and Everard Goodman Faculty of Life Sciences

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

This study was undertaken in order to assess the functional role of acetylcholinesterase (AChE) in cultures of chick skeletal muscle cells. Cultures of skeletal myotubes were prepared by mechanical dissociation of limb muscle removed from 11-day-old chick embryos and plating at a concentration of 0.8 × 10⁶ cells/ml. Cultures incubated for 4-10 days were used for electrophysiological studies with intracellular microelectrodes. Individual myotubes differed with respect to the time course of repolarization following depolarization by acetylcholine (ACh), some cells repolarizing within 2-3 min and others only after 8-10 min. Physostigmine (10^-8-10^-6 M) prolonged or sometimes completely prevented repolarization following ACh-induced depolarization. These results demonstrate that hydrolysis of ACh by AChE in cultured chick skeletal myotubes plays an important role in the repolarization of these cells following ACh-induced depolarization.

Original language	English
Pages (from-to)	338-341
Number of pages	4
Journal	Brain Research
Volume	289
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(83)90038-0
State	Published - 19 Dec 1983

Bibliographical note

Funding Information:
This work was supporteidn part by fundsf romthe ResearchA uthorityB ar-IlanU niversityW. e wish to thank Ms. Asia Bek for preparatioonf musclec ul-tures.

Funding

This work was supporteidn part by fundsf romthe ResearchA uthorityB ar-IlanU niversityW. e wish to thank Ms. Asia Bek for preparatioonf musclec ul-tures.

Funders	Funder number
ResearchA uthorityB ar-IlanU

Keywords

acetylcholine receptor
acetylcholinesterase
desensitization
muscle cultures

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10.1016/0006-8993(83)90038-0

Cite this

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title = "Evidence for a functional role of acetylcholinesterase in cultured chick myotubes",

abstract = "This study was undertaken in order to assess the functional role of acetylcholinesterase (AChE) in cultures of chick skeletal muscle cells. Cultures of skeletal myotubes were prepared by mechanical dissociation of limb muscle removed from 11-day-old chick embryos and plating at a concentration of 0.8 × 106 cells/ml. Cultures incubated for 4-10 days were used for electrophysiological studies with intracellular microelectrodes. Individual myotubes differed with respect to the time course of repolarization following depolarization by acetylcholine (ACh), some cells repolarizing within 2-3 min and others only after 8-10 min. Physostigmine (10-8-10-6 M) prolonged or sometimes completely prevented repolarization following ACh-induced depolarization. These results demonstrate that hydrolysis of ACh by AChE in cultured chick skeletal myotubes plays an important role in the repolarization of these cells following ACh-induced depolarization.",

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author = "Sampson, {S. R.} and Tamar Babila and Disatnik, {Marie Helene} and A. Shainberg and Esther Yoles",

note = "Funding Information: This work was supporteidn part by fundsf romthe ResearchA uthorityB ar-IlanU niversityW. e wish to thank Ms. Asia Bek for preparatioonf musclec ul-tures.",

year = "1983",

month = dec,

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doi = "10.1016/0006-8993(83)90038-0",

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AU - Disatnik, Marie Helene

AU - Shainberg, A.

AU - Yoles, Esther

N1 - Funding Information: This work was supporteidn part by fundsf romthe ResearchA uthorityB ar-IlanU niversityW. e wish to thank Ms. Asia Bek for preparatioonf musclec ul-tures.

PY - 1983/12/19

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N2 - This study was undertaken in order to assess the functional role of acetylcholinesterase (AChE) in cultures of chick skeletal muscle cells. Cultures of skeletal myotubes were prepared by mechanical dissociation of limb muscle removed from 11-day-old chick embryos and plating at a concentration of 0.8 × 106 cells/ml. Cultures incubated for 4-10 days were used for electrophysiological studies with intracellular microelectrodes. Individual myotubes differed with respect to the time course of repolarization following depolarization by acetylcholine (ACh), some cells repolarizing within 2-3 min and others only after 8-10 min. Physostigmine (10-8-10-6 M) prolonged or sometimes completely prevented repolarization following ACh-induced depolarization. These results demonstrate that hydrolysis of ACh by AChE in cultured chick skeletal myotubes plays an important role in the repolarization of these cells following ACh-induced depolarization.

AB - This study was undertaken in order to assess the functional role of acetylcholinesterase (AChE) in cultures of chick skeletal muscle cells. Cultures of skeletal myotubes were prepared by mechanical dissociation of limb muscle removed from 11-day-old chick embryos and plating at a concentration of 0.8 × 106 cells/ml. Cultures incubated for 4-10 days were used for electrophysiological studies with intracellular microelectrodes. Individual myotubes differed with respect to the time course of repolarization following depolarization by acetylcholine (ACh), some cells repolarizing within 2-3 min and others only after 8-10 min. Physostigmine (10-8-10-6 M) prolonged or sometimes completely prevented repolarization following ACh-induced depolarization. These results demonstrate that hydrolysis of ACh by AChE in cultured chick skeletal myotubes plays an important role in the repolarization of these cells following ACh-induced depolarization.

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Evidence for a functional role of acetylcholinesterase in cultured chick myotubes

Abstract

Bibliographical note

Funding

Keywords

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