Estrogen receptor binding material in blood of patients after clomiphene citrate administration: Determination by a radioreceptor assay

A. Geier, B. Lunenfeld, C. Pariente, S. Kotev-Emeth, A. Shadmi, E. Kokia, J. Blankstein

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

The aim of the current study was to assess whether clomiphene citrate (CC) and/or active metabolites are present at presumed time of ovulation, nidation, or steroid-sensitive organogenesis, in serum of patients receiving CC for induction of ovulation. A radioreceptor assay, based on competitive replacement of 3H-estradiol on the rat uterus estrogen receptor, by ligands present in serum of patients after CC administration, was developed. Ligands reached maximal concentration 4 to 5 hours after a single dose of CC was administered, and declined with a half-life of 4.5 to 10 hours. In patients receiving CC on day 5 to day 9 in the cycle, ligands are still present on day 14 in the cycle and in some patients on day 22 of the cycle, but no ligands were detected 60 days after CC treatment.

Original languageEnglish
Pages (from-to)778-784
Number of pages7
JournalFertility and Sterility
Volume47
Issue number5
DOIs
StatePublished - May 1987
Externally publishedYes

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