Estimation of nonspecific lectin-mediated staining of glutaraldehyde-fixed cells

N. Gilboa-Garber, L. Mizrahi

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Lectin-mediated stainings are widely used for the visualization of carbohydrate-carrying cellular components using the electron microscope. The use of glutarladehyde-fixed cells for these stainings introduces the possibility of low nonspecific lectin-trapping by the glutaraldehyde which coats the cells. This trapping was estimated by means of peroxidase-binding to human leukocytes, Tetrahymena pyriformis and Escherichia coli cells and was shown to be prevented by rinsing the glutaraldehyde-fixed cells in an amino acid solution before exposure to the lectin.

Original languageEnglish
Pages (from-to)1416-1417
Number of pages2
JournalExperientia
Volume36
Issue number12
DOIs
StatePublished - 15 Dec 1980

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