TY - JOUR
T1 - ESR detection of 1O2 reveals enhanced redox activity in illuminated cell cultures
AU - Lavi, Ronit
AU - Sinyakov, Michael
AU - Samuni, Amran
AU - Shatz, Smadar
AU - Friedmann, Harry
AU - Shainberg, Asher
AU - Breitbart, Haim
AU - Lubart, Rachel
PY - 2004/9
Y1 - 2004/9
N2 - Low-energy visible light (LEVL) has previously been found to modulate various processes in different biological systems. One explanation for the stimulatory effect of LEVL is light-induced reactive oxygen species formation. In the present study, both sperm and skin cells were illuminated with LEVL and were found to generate singlet oxygen (1O2). The detection of 1O2 was performed using a trapping probe, 2,2,6,6-tetramethyl-4-piperidone, coupled with electron paramagnetic resonance spectroscopy. In addition, we have shown that, together with 1O2 generation, LEVL illumination increases the reductive capacity of the cells, which explains the difficulties encountered in 1O2 detection. The potential of visible light to change the cellular redox state may explain the recently observed biostimulative effects exerted by LEVL.
AB - Low-energy visible light (LEVL) has previously been found to modulate various processes in different biological systems. One explanation for the stimulatory effect of LEVL is light-induced reactive oxygen species formation. In the present study, both sperm and skin cells were illuminated with LEVL and were found to generate singlet oxygen (1O2). The detection of 1O2 was performed using a trapping probe, 2,2,6,6-tetramethyl-4-piperidone, coupled with electron paramagnetic resonance spectroscopy. In addition, we have shown that, together with 1O2 generation, LEVL illumination increases the reductive capacity of the cells, which explains the difficulties encountered in 1O2 detection. The potential of visible light to change the cellular redox state may explain the recently observed biostimulative effects exerted by LEVL.
KW - EPR
KW - Light
KW - Photobiostimulation
KW - Singlet oxygen
KW - TEMP
UR - http://www.scopus.com/inward/record.url?scp=4243145704&partnerID=8YFLogxK
U2 - 10.1080/1071576010001642646
DO - 10.1080/1071576010001642646
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C2 - 15621706
AN - SCOPUS:4243145704
SN - 1071-5762
VL - 38
SP - 893
EP - 902
JO - Free Radical Research
JF - Free Radical Research
IS - 9
ER -