Erythrina Lectins Detect the H/HI Blood Groups

Dvora Sudakevitz; Nechama Gilboa-Garber; Cyril Levene; Ruth Sela; Lokesh Bhattacharyya

doi:10.1016/s0934-8840(11)80298-7

Erythrina Lectins Detect the H/HI Blood Groups

Dvora Sudakevitz
, Nechama Gilboa-Garber
, Cyril Levene
, Ruth Sela
, Lokesh Bhattacharyya

Bar-Ilan University
Ministry of Health, Israel
Albert Einstein College of Medicine

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The lectin purified from Erythrina corallodendron seeds which binds N-acetyllactosamine > N-acetyl-D-galactosamine > α and β galactosides > D-galactose was examined for its ABO(H) blood group specificity. It has been shown that this lectin causes the strongest hemagglutination of O(H) and weakest of Oh(Bombay) red blood cells, and interacts with the H antigen in association with the I antigen. The reactions of Erythrina corallodendron and Erythrina indica lectins (which are similar in sugar specificity) with erythrocytes of different ABO(H) and Ii blood groups (the I bloods were all from adults and the i from either cord or adult bloods) revealed the following order of activity: O(H)I > A₂ I > O(H)i adult > A₂BI > BI > O(H)i cord > A₁I > A₁i adult > Bi cord > A₁BI > Ai CQrd > ABi cord > Ohl. The Erythrina indica lectin showed a lower differentiation between the agglutination of O(H) and Oh erythrocytes. Both Erythrina lectins exhibited H/HI blood group preference but were not inhibited by the saliva from ABO(H) “secretors”. Thus they may be classified with the Cytisus sessilifolius, Lotus tetragonolobus and Laburnum alpinum lectins which are inhibited by lactose but not by H blood group substances in secretions.

Original language	English
Pages (from-to)	343-350
Number of pages	8
Journal	Zentralblatt fur Bakteriologie
Volume	275
Issue number	3
DOIs	https://doi.org/10.1016/s0934-8840(11)80298-7
State	Published - Aug 1991

Bibliographical note

Funding Information:
Acknowledgements. This research was supported by the Health Center Foundation of the Bar-Han University. The authors thank Karen Gekker for technical assistance, Drs. P. Moores and Brian Gibbs from the Rep. of South Africa for the donation of OH (Bombay) bloods used in this study, Dr.]. Jorgensen from Denmark for Oi red blood cells, Mrs. Ella Gindi and Mrs. Tamar Anker for the graphic and photographic representation of the results and Mrs. Avrille Goldreich for the typing of the manuscript.

Funding

Acknowledgements. This research was supported by the Health Center Foundation of the Bar-Han University. The authors thank Karen Gekker for technical assistance, Drs. P. Moores and Brian Gibbs from the Rep. of South Africa for the donation of OH (Bombay) bloods used in this study, Dr.]. Jorgensen from Denmark for Oi red blood cells, Mrs. Ella Gindi and Mrs. Tamar Anker for the graphic and photographic representation of the results and Mrs. Avrille Goldreich for the typing of the manuscript.

Funders
Health Center Foundation of the Bar-Han University

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10.1016/s0934-8840(11)80298-7

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title = "Erythrina Lectins Detect the H/HI Blood Groups",

abstract = "The lectin purified from Erythrina corallodendron seeds which binds N-acetyllactosamine > N-acetyl-D-galactosamine > α and β galactosides > D-galactose was examined for its ABO(H) blood group specificity. It has been shown that this lectin causes the strongest hemagglutination of O(H) and weakest of Oh(Bombay) red blood cells, and interacts with the H antigen in association with the I antigen. The reactions of Erythrina corallodendron and Erythrina indica lectins (which are similar in sugar specificity) with erythrocytes of different ABO(H) and Ii blood groups (the I bloods were all from adults and the i from either cord or adult bloods) revealed the following order of activity: O(H)I > A2 I > O(H)i adult > A2BI > BI > O(H)i cord > A1I > A1i adult > Bi cord > A1BI > Ai CQrd > ABi cord > Ohl. The Erythrina indica lectin showed a lower differentiation between the agglutination of O(H) and Oh erythrocytes. Both Erythrina lectins exhibited H/HI blood group preference but were not inhibited by the saliva from ABO(H) “secretors”. Thus they may be classified with the Cytisus sessilifolius, Lotus tetragonolobus and Laburnum alpinum lectins which are inhibited by lactose but not by H blood group substances in secretions.",

author = "Dvora Sudakevitz and Nechama Gilboa-Garber and Cyril Levene and Ruth Sela and Lokesh Bhattacharyya",

note = "Funding Information: Acknowledgements. This research was supported by the Health Center Foundation of the Bar-Han University. The authors thank Karen Gekker for technical assistance, Drs. P. Moores and Brian Gibbs from the Rep. of South Africa for the donation of OH (Bombay) bloods used in this study, Dr.]. Jorgensen from Denmark for Oi red blood cells, Mrs. Ella Gindi and Mrs. Tamar Anker for the graphic and photographic representation of the results and Mrs. Avrille Goldreich for the typing of the manuscript.",

year = "1991",

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AU - Sela, Ruth

AU - Bhattacharyya, Lokesh

N1 - Funding Information: Acknowledgements. This research was supported by the Health Center Foundation of the Bar-Han University. The authors thank Karen Gekker for technical assistance, Drs. P. Moores and Brian Gibbs from the Rep. of South Africa for the donation of OH (Bombay) bloods used in this study, Dr.]. Jorgensen from Denmark for Oi red blood cells, Mrs. Ella Gindi and Mrs. Tamar Anker for the graphic and photographic representation of the results and Mrs. Avrille Goldreich for the typing of the manuscript.

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N2 - The lectin purified from Erythrina corallodendron seeds which binds N-acetyllactosamine > N-acetyl-D-galactosamine > α and β galactosides > D-galactose was examined for its ABO(H) blood group specificity. It has been shown that this lectin causes the strongest hemagglutination of O(H) and weakest of Oh(Bombay) red blood cells, and interacts with the H antigen in association with the I antigen. The reactions of Erythrina corallodendron and Erythrina indica lectins (which are similar in sugar specificity) with erythrocytes of different ABO(H) and Ii blood groups (the I bloods were all from adults and the i from either cord or adult bloods) revealed the following order of activity: O(H)I > A2 I > O(H)i adult > A2BI > BI > O(H)i cord > A1I > A1i adult > Bi cord > A1BI > Ai CQrd > ABi cord > Ohl. The Erythrina indica lectin showed a lower differentiation between the agglutination of O(H) and Oh erythrocytes. Both Erythrina lectins exhibited H/HI blood group preference but were not inhibited by the saliva from ABO(H) “secretors”. Thus they may be classified with the Cytisus sessilifolius, Lotus tetragonolobus and Laburnum alpinum lectins which are inhibited by lactose but not by H blood group substances in secretions.

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Erythrina Lectins Detect the H/HI Blood Groups

Abstract

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