Eradication of Acinetobacter baumannii by photosensitized agents in vitro

Yeshayahu Nitzan; Anat Balzam-Sudakevitz; Helena Ashkenazi

doi:10.1016/s1011-1344(98)00073-6

Eradication of Acinetobacter baumannii by photosensitized agents in vitro

Yeshayahu Nitzan, Anat Balzam-Sudakevitz, Helena Ashkenazi

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

102 Scopus citations

Abstract

The photodynamic effects of photosensitizers on Acinetobacter baumannii were studied. These Gram negative bacteria have recently been implicated in various infections, mainly acquired in hospitals. They have outstanding characteristics of multidrug high resistance to antimicrobial agents. The best photodynamic effect was obtained when A. baumannii cultures were treated with light activated deuteroporphyrin (Dp) at a concentration of 34 μmoles l^-1 and polymyxin nonapeptide (PMNP) at a concentration of 200 μmoles l^-1. At these concentrations the culture in brain heart infusion (BHI) broth was found to be sterile after 1 h of treatment. Some inhibition was also obtained under the same conditions with Cd-texaphyrin (Cd-Tx) in the presence of PMNP. Treatment with various other photosensitizers in the presence of PMNP exhibited only marginal antibacterial activity. The cationic photosensitizer tetra-methylpyridyl porphine (TMPyP) did not exhibit any photodynamic effect on A. baumannii when illuminated during its growth in BHI broth. Bacteria grown in nutrient broth or suspended in saline and trrated by TMPyP resulted in a significant photoinactivation by the sensitizer alone even in the absence of PMNP. It was found that a high concentration of the proteins present in BHI or in serum prevent TMPyP from acting as a photosensitizer against A. baumannii. Bovine serum albumin at the same high protein concentration prevents Dp (in the presence of PMNP) to act as a photosensitizer. The amionic photosensitizer tetra-sulfonatopheny] porphine (TPPS₄) did not show any photodynamic effect in high or low protein media. In this study it was found that despite the high resistance of the Acinetobacter baumannii to antibiotics, these bacteria can be significantly photoinactivated by treatment with either Dp + PMNP or TMPyP in low protein content environments. When the protein concentration is high, photoinactivation efficiency depends on the type of protein present in the medium.

Original language	English
Pages (from-to)	211-218
Number of pages	8
Journal	Journal of Photochemistry and Photobiology B: Biology
Volume	42
Issue number	3
DOIs	https://doi.org/10.1016/s1011-1344(98)00073-6
State	Published - Mar 1998

Bibliographical note

Funding Information:
The authors would like to thank Mrs R. Dror for excellent technical assistance. This work was supported in part by a grant from the Health Sciences Research Center Funds (to Y.N.).

Funding

The authors would like to thank Mrs R. Dror for excellent technical assistance. This work was supported in part by a grant from the Health Sciences Research Center Funds (to Y.N.).

Funders	Funder number
Health Sciences Research Center Funds

Keywords

Actinetobacter
Cationic porphyrins
Deuteroporphyrin
Gram-negative bacteria
Photosensitization
Polymyxin nonapeptide

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10.1016/s1011-1344(98)00073-6

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title = "Eradication of Acinetobacter baumannii by photosensitized agents in vitro",

abstract = "The photodynamic effects of photosensitizers on Acinetobacter baumannii were studied. These Gram negative bacteria have recently been implicated in various infections, mainly acquired in hospitals. They have outstanding characteristics of multidrug high resistance to antimicrobial agents. The best photodynamic effect was obtained when A. baumannii cultures were treated with light activated deuteroporphyrin (Dp) at a concentration of 34 μmoles l-1 and polymyxin nonapeptide (PMNP) at a concentration of 200 μmoles l-1. At these concentrations the culture in brain heart infusion (BHI) broth was found to be sterile after 1 h of treatment. Some inhibition was also obtained under the same conditions with Cd-texaphyrin (Cd-Tx) in the presence of PMNP. Treatment with various other photosensitizers in the presence of PMNP exhibited only marginal antibacterial activity. The cationic photosensitizer tetra-methylpyridyl porphine (TMPyP) did not exhibit any photodynamic effect on A. baumannii when illuminated during its growth in BHI broth. Bacteria grown in nutrient broth or suspended in saline and trrated by TMPyP resulted in a significant photoinactivation by the sensitizer alone even in the absence of PMNP. It was found that a high concentration of the proteins present in BHI or in serum prevent TMPyP from acting as a photosensitizer against A. baumannii. Bovine serum albumin at the same high protein concentration prevents Dp (in the presence of PMNP) to act as a photosensitizer. The amionic photosensitizer tetra-sulfonatopheny] porphine (TPPS4) did not show any photodynamic effect in high or low protein media. In this study it was found that despite the high resistance of the Acinetobacter baumannii to antibiotics, these bacteria can be significantly photoinactivated by treatment with either Dp + PMNP or TMPyP in low protein content environments. When the protein concentration is high, photoinactivation efficiency depends on the type of protein present in the medium.",

keywords = "Actinetobacter, Cationic porphyrins, Deuteroporphyrin, Gram-negative bacteria, Photosensitization, Polymyxin nonapeptide",

author = "Yeshayahu Nitzan and Anat Balzam-Sudakevitz and Helena Ashkenazi",

note = "Funding Information: The authors would like to thank Mrs R. Dror for excellent technical assistance. This work was supported in part by a grant from the Health Sciences Research Center Funds (to Y.N.).",

year = "1998",

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AU - Balzam-Sudakevitz, Anat

AU - Ashkenazi, Helena

N1 - Funding Information: The authors would like to thank Mrs R. Dror for excellent technical assistance. This work was supported in part by a grant from the Health Sciences Research Center Funds (to Y.N.).

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N2 - The photodynamic effects of photosensitizers on Acinetobacter baumannii were studied. These Gram negative bacteria have recently been implicated in various infections, mainly acquired in hospitals. They have outstanding characteristics of multidrug high resistance to antimicrobial agents. The best photodynamic effect was obtained when A. baumannii cultures were treated with light activated deuteroporphyrin (Dp) at a concentration of 34 μmoles l-1 and polymyxin nonapeptide (PMNP) at a concentration of 200 μmoles l-1. At these concentrations the culture in brain heart infusion (BHI) broth was found to be sterile after 1 h of treatment. Some inhibition was also obtained under the same conditions with Cd-texaphyrin (Cd-Tx) in the presence of PMNP. Treatment with various other photosensitizers in the presence of PMNP exhibited only marginal antibacterial activity. The cationic photosensitizer tetra-methylpyridyl porphine (TMPyP) did not exhibit any photodynamic effect on A. baumannii when illuminated during its growth in BHI broth. Bacteria grown in nutrient broth or suspended in saline and trrated by TMPyP resulted in a significant photoinactivation by the sensitizer alone even in the absence of PMNP. It was found that a high concentration of the proteins present in BHI or in serum prevent TMPyP from acting as a photosensitizer against A. baumannii. Bovine serum albumin at the same high protein concentration prevents Dp (in the presence of PMNP) to act as a photosensitizer. The amionic photosensitizer tetra-sulfonatopheny] porphine (TPPS4) did not show any photodynamic effect in high or low protein media. In this study it was found that despite the high resistance of the Acinetobacter baumannii to antibiotics, these bacteria can be significantly photoinactivated by treatment with either Dp + PMNP or TMPyP in low protein content environments. When the protein concentration is high, photoinactivation efficiency depends on the type of protein present in the medium.

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Eradication of Acinetobacter baumannii by photosensitized agents in vitro

Abstract

Bibliographical note

Funding

Keywords

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