EPR analysis of radicals generated in ultrasound-assisted lipoplasty simulated environment

Moris Topaz, Menachem Motiei, Aharon Gedanken, Dan Meyerstein, Naomi Meyerstein

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


The generation of various radicals by application of continuous wave (CW) high-intensity ultrasound energy (HIUE) to an aqueous biologic medium containing spin traps, under conditions simulating ultrasound-assisted lipoplasty (UAL), was demonstrated by EPR spectroscopy. The addition of water- soluble antioxidants, ascorbic acid and glutathione to the wetting solution substantially reduces the levels of hydroxyl radicals in the sonicated medium. These findings provide direct evidence for the generation of cavitation in the simulated intercellular environment, corroborating previous data, and pointing out that generation of transient cavitation in clinical UAL and other therapeutic and surgical applications of ultrasound is possible. The findings indicate that the effect of transient cavitation in aqueous biologic media may be similar to the effects of ionizing radiation, and raise the question of the long-term biosafety of the use of CW HIUE in UAL. The introduction of biocompatible water-soluble antioxidants to the sonicated medium may be utilized to suppress accumulation of radicals and reduce their possible adverse effects. (E-mail: topazmd@netvision.net.il)

Original languageEnglish
Pages (from-to)851-859
Number of pages9
JournalUltrasound in Medicine and Biology
Issue number6
StatePublished - Jun 2001


  • Antioxidants
  • Cavitation
  • Cavitation safety index
  • EPR spectroscopy
  • High-intensity ultrasound energy (HIUE)
  • Long-term biosafety
  • Radicals
  • Ultrasound-assisted lipoplasty (UAL)


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