Enhancing effect of cyclosporins A and G on bone marrow colony formation

Y. Kalechman, B. Sredni, R. R. Caspi, R. B. Nussenblatt

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7 Scopus citations


The development of the number of colonies (cell colony-forming units, CFU-C) in soft agar from normal mouse bone marrow (BM) cells was enhanced 60% when total bone marrow cells (BMC) were preincubated for 1 h with either cyclosporin A (CsA) or cyclosporin G (CsG) before plating. Using cell fractionation techniques we found that the removal of macrophages enhanced CFU-C in noncyclosporin-treated BM and that cyclosporins mediated an additional enhancing effect. A similar enhancing effect of CFU-C in noncyclosporin-treated BM was obtained by depleting it of total T cells or Lyt-2.2+ cells. However, CFU-C growth in the residual BM population was no longer enhanced by cyclosporin. Conversely, removal of Lyt-1.2+ cells did not enhance CFU-C in noncyclosporin-treated BM, but the CFU-C in this population were enhanced by cyclosporin treatment. These results suggest that CsA and CsG can increase the cloning efficiency of normal mouse BMC, possibly by inhibiting an endogenous Lyt-2.2+ suppressor cell.

Original languageEnglish
Pages (from-to)1104-1107
Number of pages4
JournalExperimental Hematology
Issue number10
StatePublished - 1990


  • CFU-C
  • Cyclosporins


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