Enhancement of the rate of the β-elimination of phosphate from radicals derived from glycerol-2-phosphate by Cu(I)-phenanthroline. A pulse radiolysis study

Sara Goldstein, Gidon Czapski, Haim Cohen, Dan Meyerstein

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Hydroxyl radicals abstract hydrogen atoms from glycerol-2-phosphate with a specific rate constant of (7.0 ± 1.5) × 108 M-1s-1 forming the β-phospho radical as the major product. At physiological pH this radical undergoes a β-phosphate elimination with a rate constant ≤ 1 × 103 s-1. The β-phospho radical reacts with CU(I)-phenanthroline to produce an unstable transient with a metal-carbon σ-bond which has an absorbance similar to that of the cuprous phenanthroline complex in the visible region. This intermediate decomposes via a β-elimination of phosphate with a rate constant of 1.0 ± 1.5) × 104s-1, which was independent of the acidity in the pH range 4-9.

Original languageEnglish
Pages (from-to)371-379
Number of pages9
JournalFree Radical Biology and Medicine
Volume9
Issue number5
DOIs
StatePublished - 1990
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgements -- This work was supported in part by grants from the Israel Academy of Sciences, the Planning and Granting Committee of the Council of Higher Education, The Israel USA Binational Science Foundation, The Israel Atomic Energy Commission, and the Council for Tobacco Research.

Funding

Acknowledgements -- This work was supported in part by grants from the Israel Academy of Sciences, the Planning and Granting Committee of the Council of Higher Education, The Israel USA Binational Science Foundation, The Israel Atomic Energy Commission, and the Council for Tobacco Research.

FundersFunder number
Council for Tobacco Research
Council of Higher Education, The Israel USA Binational Science Foundation
Israel Academy of Sciences and Humanities
Israel Atomic Energy Commission

    Keywords

    • Aliphatic radical
    • Cu(I)-phenonanthroline
    • DNA damage
    • Free radicals
    • Radiation
    • β-elimination

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