Hydroxyl radicals abstract hydrogen atoms from glycerol-2-phosphate with a specific rate constant of (7.0 ± 1.5) × 108 M-1s-1 forming the β-phospho radical as the major product. At physiological pH this radical undergoes a β-phosphate elimination with a rate constant ≤ 1 × 103 s-1. The β-phospho radical reacts with CU(I)-phenanthroline to produce an unstable transient with a metal-carbon σ-bond which has an absorbance similar to that of the cuprous phenanthroline complex in the visible region. This intermediate decomposes via a β-elimination of phosphate with a rate constant of 1.0 ± 1.5) × 104s-1, which was independent of the acidity in the pH range 4-9.
Bibliographical noteFunding Information:
Acknowledgements -- This work was supported in part by grants from the Israel Academy of Sciences, the Planning and Granting Committee of the Council of Higher Education, The Israel USA Binational Science Foundation, The Israel Atomic Energy Commission, and the Council for Tobacco Research.
- Aliphatic radical
- DNA damage
- Free radicals