Enhancement of bone defect healing in old rats by TGF-β and IGF-1

I. Blumenfeld; S. Srouji; Y. Lanir; D. Laufer; E. Livne

doi:10.1016/s0531-5565(01)00215-7

Enhancement of bone defect healing in old rats by TGF-β and IGF-1

I. Blumenfeld, S. Srouji, Y. Lanir, D. Laufer, E. Livne

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

Bone defects are often created in order to repair bone pathologies. In the aging population, the healing of such defects is very limited. Bone healing in aging depends on the availability of various hormone and growth factors. The ability of growth factors to enhance bone formation in femoral defects in old rats was tested. Bone defects were induced in femurs of old rats. A single dose of transforming growth factor-β (TGF-β), IGF-1, TGF-β + IGF-1 or saline was inserted in the defect and bones were tested after 2 and 4 weeks. Radiology revealed that mineralization appeared in the 2 weeks group in defects treated with TGF-β and in defects treated with TGF-β, TGF-β + IGF-1 in the 4 weeks groups. Computerized tomography (CT) coronal and axial images revealed that 4 weeks after treatment with TGF-β + IGF-1, a complete bone bridge was observed. Morphology revealed that these defects were filled with trabecular bone. A less pronounced bone healing was observed after TGF-β or IGF-1, while control specimens revealed partial healing of the bone defect. Biomechanical tests indicated that treatment with TGF-β, IGF-1 or TGF-β + IGF-1 resulted in a significant increase of bone bending rigidity compared to control in the 4 weeks group and that TGF-β + IGF-1 was the most inductive in this respect. The ability to induce bone healing in aging by TGF-β + IGF-1 is of a great clinical importance for restoration of bone strength and biomechanical properties of bone defects in aging.

Original language	English
Pages (from-to)	553-565
Number of pages	13
Journal	Experimental Gerontology
Volume	37
Issue number	4
DOIs	https://doi.org/10.1016/s0531-5565(01)00215-7
State	Published - Apr 2002
Externally published	Yes

Bibliographical note

Funding Information:
The present study was supported by Technion VPR grants No. 181-819, 180-334. The authors wish to acknowledge the excellent technical assistance of Mr A. Sverdlik and Mrs A. Ziskind.

Funding

The present study was supported by Technion VPR grants No. 181-819, 180-334. The authors wish to acknowledge the excellent technical assistance of Mr A. Sverdlik and Mrs A. Ziskind.

Funders	Funder number
Technion-Israel Institute of Technology	181-819, 180-334

Keywords

Aging
Bone defect
Growth factors

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10.1016/s0531-5565(01)00215-7

Cite this

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title = "Enhancement of bone defect healing in old rats by TGF-β and IGF-1",

abstract = "Bone defects are often created in order to repair bone pathologies. In the aging population, the healing of such defects is very limited. Bone healing in aging depends on the availability of various hormone and growth factors. The ability of growth factors to enhance bone formation in femoral defects in old rats was tested. Bone defects were induced in femurs of old rats. A single dose of transforming growth factor-β (TGF-β), IGF-1, TGF-β + IGF-1 or saline was inserted in the defect and bones were tested after 2 and 4 weeks. Radiology revealed that mineralization appeared in the 2 weeks group in defects treated with TGF-β and in defects treated with TGF-β, TGF-β + IGF-1 in the 4 weeks groups. Computerized tomography (CT) coronal and axial images revealed that 4 weeks after treatment with TGF-β + IGF-1, a complete bone bridge was observed. Morphology revealed that these defects were filled with trabecular bone. A less pronounced bone healing was observed after TGF-β or IGF-1, while control specimens revealed partial healing of the bone defect. Biomechanical tests indicated that treatment with TGF-β, IGF-1 or TGF-β + IGF-1 resulted in a significant increase of bone bending rigidity compared to control in the 4 weeks group and that TGF-β + IGF-1 was the most inductive in this respect. The ability to induce bone healing in aging by TGF-β + IGF-1 is of a great clinical importance for restoration of bone strength and biomechanical properties of bone defects in aging.",

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note = "Funding Information: The present study was supported by Technion VPR grants No. 181-819, 180-334. The authors wish to acknowledge the excellent technical assistance of Mr A. Sverdlik and Mrs A. Ziskind.",

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Enhancement of bone defect healing in old rats by TGF-β and IGF-1

Abstract

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Keywords

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