Enhancement mechanism of fluorescence intensity in presence of plasmonic nanoparticles

Sumana Das; Ramakrishna Vasireddi; Krishna Harika Villa; Brahmanandam Javvaji; Gopalkrishna M. Hegde; D. Roy Mahapatra

doi:10.1117/12.2207565

Enhancement mechanism of fluorescence intensity in presence of plasmonic nanoparticles

Sumana Das, Ramakrishna Vasireddi, Krishna Harika Villa, Brahmanandam Javvaji, Gopalkrishna M. Hegde, D. Roy Mahapatra

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Scopus citations

Abstract

The emission intensity of fluorophore molecule may change in presence of strong plasmon field induced by nanoparticles. The enhancement intensity is optimized through selective clustering or functionalization of nanoparticles in closed vicinity of fluorophore. Our study is aimed at understanding the enhancement mechanism of fluorescence intensity in presence of gold nanoparticles to utilize it in molecular sensing and in situ imaging in the microfluidic lab-on-chip device. Related phenomena are studied in situ in a microfluidic channel via fluorescence imaging. Detailed analysis is carried out to understand the possible mechanism of enhancement of fluorescence due to nanoparticles. In the present experimental study we show that SYTO9 fluorescence intensity increased in presence of Au nanoparticles of ~20 nm diameter. The fluorescence intensity is 20 time more compared to that in absence of Au nanoparticles. The enhancement of fluorescence intensity is attributed to the plasmonic resonance of Au nanoparticle at around the fluorescence emission wavelength. Underlying fundamental mechanism via dipole interaction model is explored for quantitative correlation of plasmonic enhancement properties.

Original language	English
Title of host publication	Biophotonics Japan 2015
Editors	Takashige Omatsu, Yasuyuki Ozeki, Seigo Ohno, Yoshio Hayasaki, Yusuke Ogura
Publisher	SPIE
ISBN (Electronic)	9781510600270
DOIs	https://doi.org/10.1117/12.2207565
State	Published - 2015
Externally published	Yes
Event	Biophotonics Japan 2015 - Tokyo, Japan Duration: 27 Oct 2015 → 28 Oct 2015

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	9792
ISSN (Print)	1605-7422

Conference

Conference	Biophotonics Japan 2015
Country/Territory	Japan
City	Tokyo
Period	27/10/15 → 28/10/15

Bibliographical note

Publisher Copyright:
© 2015 COPYRIGHT SPIE.

Keywords

dipole
fluorescence
nanoparticle
optical transmission
plasmon
resonance

Access to Document

10.1117/12.2207565

Cite this

Das, S., Vasireddi, R., Harika Villa, K., Javvaji, B., Hegde, G. M., & Roy Mahapatra, D. (2015). Enhancement mechanism of fluorescence intensity in presence of plasmonic nanoparticles. In T. Omatsu, Y. Ozeki, S. Ohno, Y. Hayasaki, & Y. Ogura (Eds.), Biophotonics Japan 2015 [979208] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9792). SPIE. https://doi.org/10.1117/12.2207565

@inproceedings{74661e03d4da4b56ab9bb6ac5410807c,

title = "Enhancement mechanism of fluorescence intensity in presence of plasmonic nanoparticles",

abstract = "The emission intensity of fluorophore molecule may change in presence of strong plasmon field induced by nanoparticles. The enhancement intensity is optimized through selective clustering or functionalization of nanoparticles in closed vicinity of fluorophore. Our study is aimed at understanding the enhancement mechanism of fluorescence intensity in presence of gold nanoparticles to utilize it in molecular sensing and in situ imaging in the microfluidic lab-on-chip device. Related phenomena are studied in situ in a microfluidic channel via fluorescence imaging. Detailed analysis is carried out to understand the possible mechanism of enhancement of fluorescence due to nanoparticles. In the present experimental study we show that SYTO9 fluorescence intensity increased in presence of Au nanoparticles of ~20 nm diameter. The fluorescence intensity is 20 time more compared to that in absence of Au nanoparticles. The enhancement of fluorescence intensity is attributed to the plasmonic resonance of Au nanoparticle at around the fluorescence emission wavelength. Underlying fundamental mechanism via dipole interaction model is explored for quantitative correlation of plasmonic enhancement properties.",

keywords = "dipole, fluorescence, nanoparticle, optical transmission, plasmon, resonance",

author = "Sumana Das and Ramakrishna Vasireddi and {Harika Villa}, Krishna and Brahmanandam Javvaji and Hegde, {Gopalkrishna M.} and {Roy Mahapatra}, D.",

note = "Publisher Copyright: {\textcopyright} 2015 COPYRIGHT SPIE.; Biophotonics Japan 2015 ; Conference date: 27-10-2015 Through 28-10-2015",

year = "2015",

doi = "10.1117/12.2207565",

language = "אנגלית",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Takashige Omatsu and Yasuyuki Ozeki and Seigo Ohno and Yoshio Hayasaki and Yusuke Ogura",

booktitle = "Biophotonics Japan 2015",

address = "ארצות הברית",

}

Das, S, Vasireddi, R, Harika Villa, K, Javvaji, B, Hegde, GM & Roy Mahapatra, D 2015, Enhancement mechanism of fluorescence intensity in presence of plasmonic nanoparticles. in T Omatsu, Y Ozeki, S Ohno, Y Hayasaki & Y Ogura (eds), Biophotonics Japan 2015., 979208, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9792, SPIE, Biophotonics Japan 2015, Tokyo, Japan, 27/10/15. https://doi.org/10.1117/12.2207565

Enhancement mechanism of fluorescence intensity in presence of plasmonic nanoparticles. / Das, Sumana; Vasireddi, Ramakrishna; Harika Villa, Krishna et al.
Biophotonics Japan 2015. ed. / Takashige Omatsu; Yasuyuki Ozeki; Seigo Ohno; Yoshio Hayasaki; Yusuke Ogura. SPIE, 2015. 979208 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9792).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Enhancement mechanism of fluorescence intensity in presence of plasmonic nanoparticles

AU - Das, Sumana

AU - Vasireddi, Ramakrishna

AU - Harika Villa, Krishna

AU - Javvaji, Brahmanandam

AU - Hegde, Gopalkrishna M.

AU - Roy Mahapatra, D.

PY - 2015

Y1 - 2015

N2 - The emission intensity of fluorophore molecule may change in presence of strong plasmon field induced by nanoparticles. The enhancement intensity is optimized through selective clustering or functionalization of nanoparticles in closed vicinity of fluorophore. Our study is aimed at understanding the enhancement mechanism of fluorescence intensity in presence of gold nanoparticles to utilize it in molecular sensing and in situ imaging in the microfluidic lab-on-chip device. Related phenomena are studied in situ in a microfluidic channel via fluorescence imaging. Detailed analysis is carried out to understand the possible mechanism of enhancement of fluorescence due to nanoparticles. In the present experimental study we show that SYTO9 fluorescence intensity increased in presence of Au nanoparticles of ~20 nm diameter. The fluorescence intensity is 20 time more compared to that in absence of Au nanoparticles. The enhancement of fluorescence intensity is attributed to the plasmonic resonance of Au nanoparticle at around the fluorescence emission wavelength. Underlying fundamental mechanism via dipole interaction model is explored for quantitative correlation of plasmonic enhancement properties.

AB - The emission intensity of fluorophore molecule may change in presence of strong plasmon field induced by nanoparticles. The enhancement intensity is optimized through selective clustering or functionalization of nanoparticles in closed vicinity of fluorophore. Our study is aimed at understanding the enhancement mechanism of fluorescence intensity in presence of gold nanoparticles to utilize it in molecular sensing and in situ imaging in the microfluidic lab-on-chip device. Related phenomena are studied in situ in a microfluidic channel via fluorescence imaging. Detailed analysis is carried out to understand the possible mechanism of enhancement of fluorescence due to nanoparticles. In the present experimental study we show that SYTO9 fluorescence intensity increased in presence of Au nanoparticles of ~20 nm diameter. The fluorescence intensity is 20 time more compared to that in absence of Au nanoparticles. The enhancement of fluorescence intensity is attributed to the plasmonic resonance of Au nanoparticle at around the fluorescence emission wavelength. Underlying fundamental mechanism via dipole interaction model is explored for quantitative correlation of plasmonic enhancement properties.

KW - dipole

KW - fluorescence

KW - nanoparticle

KW - optical transmission

KW - plasmon

KW - resonance

UR - http://www.scopus.com/inward/record.url?scp=84958599392&partnerID=8YFLogxK

U2 - 10.1117/12.2207565

DO - 10.1117/12.2207565

M3 - ???researchoutput.researchoutputtypes.contributiontobookanthology.conference???

AN - SCOPUS:84958599392

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Biophotonics Japan 2015

A2 - Omatsu, Takashige

A2 - Ozeki, Yasuyuki

A2 - Ohno, Seigo

A2 - Hayasaki, Yoshio

A2 - Ogura, Yusuke

PB - SPIE

T2 - Biophotonics Japan 2015

Y2 - 27 October 2015 through 28 October 2015

ER -

Enhancement mechanism of fluorescence intensity in presence of plasmonic nanoparticles

Abstract

Publication series

Conference

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this