Enhancement mechanism of fluorescence intensity in presence of plasmonic nanoparticles

Sumana Das, Ramakrishna Vasireddi, Krishna Harika Villa, Brahmanandam Javvaji, Gopalkrishna M. Hegde, D. Roy Mahapatra

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Scopus citations


The emission intensity of fluorophore molecule may change in presence of strong plasmon field induced by nanoparticles. The enhancement intensity is optimized through selective clustering or functionalization of nanoparticles in closed vicinity of fluorophore. Our study is aimed at understanding the enhancement mechanism of fluorescence intensity in presence of gold nanoparticles to utilize it in molecular sensing and in situ imaging in the microfluidic lab-on-chip device. Related phenomena are studied in situ in a microfluidic channel via fluorescence imaging. Detailed analysis is carried out to understand the possible mechanism of enhancement of fluorescence due to nanoparticles. In the present experimental study we show that SYTO9 fluorescence intensity increased in presence of Au nanoparticles of ~20 nm diameter. The fluorescence intensity is 20 time more compared to that in absence of Au nanoparticles. The enhancement of fluorescence intensity is attributed to the plasmonic resonance of Au nanoparticle at around the fluorescence emission wavelength. Underlying fundamental mechanism via dipole interaction model is explored for quantitative correlation of plasmonic enhancement properties.

Original languageEnglish
Title of host publicationBiophotonics Japan 2015
EditorsTakashige Omatsu, Yasuyuki Ozeki, Seigo Ohno, Yoshio Hayasaki, Yusuke Ogura
ISBN (Electronic)9781510600270
StatePublished - 2015
Externally publishedYes
EventBiophotonics Japan 2015 - Tokyo, Japan
Duration: 27 Oct 201528 Oct 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceBiophotonics Japan 2015

Bibliographical note

Publisher Copyright:


  • dipole
  • fluorescence
  • nanoparticle
  • optical transmission
  • plasmon
  • resonance


Dive into the research topics of 'Enhancement mechanism of fluorescence intensity in presence of plasmonic nanoparticles'. Together they form a unique fingerprint.

Cite this