TY - JOUR
T1 - Enhanced Fluorescence Imaging of Implants Based on Polyester Copolymers in Combination With MRI
AU - Zherdeva, Victoria V.
AU - Likhov, Astemir R.
AU - Saidvaliev, Ulugbek A.
AU - Fixler, Dror
AU - Demin, Dmitry
AU - Volodina, Veronika N.
AU - Apukhtina, Uliana A.
AU - Pawar, Shweta
AU - Atuar, Bar
AU - Tuchin, Valery V.
N1 - Publisher Copyright:
© 2025 Wiley-VCH GmbH.
PY - 2025/12
Y1 - 2025/12
N2 - Nowadays, many biodegradable materials are offered for biomedical applications, but there are only a few in vivo methods for their detection and monitoring. In this work, implants based on biodegradable polyester copolymers were labeled with indocyanine green (ICG) for fluorescence imaging in combination with tissue optical clearing (TOC) and magnetic resonance imaging (MRI). The results include in vitro degradation modeling followed by in vivo imaging of copolymer samples that were subcutaneously implanted in BALB/c mice. TOC with 70% glycerol has been demonstrated to significantly improve sample visualization. The TOC efficiency parameter Q demonstrated the variability of effects correlating with the timing of follow-up in the postimplantation period. It has been shown that nonhealing wounds, peri-implantation inflammation, or fibrosis, confirmed by MRI, affect the effectiveness of TOC in the range from Q = −30% to 70%.
AB - Nowadays, many biodegradable materials are offered for biomedical applications, but there are only a few in vivo methods for their detection and monitoring. In this work, implants based on biodegradable polyester copolymers were labeled with indocyanine green (ICG) for fluorescence imaging in combination with tissue optical clearing (TOC) and magnetic resonance imaging (MRI). The results include in vitro degradation modeling followed by in vivo imaging of copolymer samples that were subcutaneously implanted in BALB/c mice. TOC with 70% glycerol has been demonstrated to significantly improve sample visualization. The TOC efficiency parameter Q demonstrated the variability of effects correlating with the timing of follow-up in the postimplantation period. It has been shown that nonhealing wounds, peri-implantation inflammation, or fibrosis, confirmed by MRI, affect the effectiveness of TOC in the range from Q = −30% to 70%.
KW - biodegradation
KW - efficiency of optical clearing
KW - fluorescence imaging
KW - glycerol
KW - indocyanine green
KW - magnetic resonance imaging
KW - multimodal in vivo imaging
KW - optical clearing
KW - polyester copolymers implants
KW - resorption
UR - https://www.scopus.com/pages/publications/85216811155
U2 - 10.1002/jbio.202400147
DO - 10.1002/jbio.202400147
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C2 - 39899887
AN - SCOPUS:85216811155
SN - 1864-063X
VL - 18
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 12
M1 - e202400147
ER -