Engraftment of human blood malignancies to the turkey embryo: A robust new in vivo model

Igor Grinberg; Arbel Reis; Avivit Ohana; Moran Taizi; Michal Cipok; Sigal Tavor; Deborah Rund; Varda R. Deutsch; Ronald S. Goldstein

doi:10.1016/j.leukres.2009.02.009

Engraftment of human blood malignancies to the turkey embryo: A robust new in vivo model

Igor Grinberg
, Arbel Reis
, Avivit Ohana
, Moran Taizi
, Michal Cipok
, Sigal Tavor
, Deborah Rund
, Varda R. Deutsch
, Ronald S. Goldstein

The Mina and Everard Goodman Faculty of Life Sciences - at Bar-Ilan University

Bar-Ilan University
Tel Aviv Sourasky Medical Center
Tel Aviv University
Hadassah University Medical Centre

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Xenografting of human blood malignancies to immunodeficient SCID mice is a powerful research tool. We evaluate here whether the immunodeficient turkey embryo can also serve as a xenograft host for human blood malignancies. Human leukemia, lymphoma and myeloma lines engrafted robustly into medullary and extramedullary tissues of turkey embryos as detected by PCR, FACS and histology in 8-10 days. Four of eleven patient AML samples also engrafted the bone marrow. Grafts of two lines responded to chemotherapy with doxorubicin. The turkey embryo therefore has the potential to be a complementary xenograft model for the study of human blood malignancies.

Original language	English
Pages (from-to)	1417-1426
Number of pages	10
Journal	Leukemia Research
Volume	33
Issue number	10
DOIs	https://doi.org/10.1016/j.leukres.2009.02.009
State	Published - Oct 2009

Bibliographical note

Funding Information:
Thanks to Tsvee Lapidot for providing the G2 cells, to Ada Refaeli for providing the HL-60 line and Galit Granot for the HBL-2 cells. Thanks to Chaya Morgenstern, Dr. Sigi Kay and Shoshana Baron for excellent technical and administrative assistance. Undergraduate interns Alexandra Polsky and Karen Levi provided invaluable assistance. This research was supported by grants by the Horowitz Foundation for Applied Biomedical Research to R.S.G. and the Israel Ministry of Health (Weinkselbaum family medical research fund), the Israel Cancer Association through a donation from Don and Donna Sherman and Caesaria Edmond Benjamin de Rothchild Foundation (V.R.D.). None of the granting agencies participated in any aspect of the study.

Funding

Thanks to Tsvee Lapidot for providing the G2 cells, to Ada Refaeli for providing the HL-60 line and Galit Granot for the HBL-2 cells. Thanks to Chaya Morgenstern, Dr. Sigi Kay and Shoshana Baron for excellent technical and administrative assistance. Undergraduate interns Alexandra Polsky and Karen Levi provided invaluable assistance. This research was supported by grants by the Horowitz Foundation for Applied Biomedical Research to R.S.G. and the Israel Ministry of Health (Weinkselbaum family medical research fund), the Israel Cancer Association through a donation from Don and Donna Sherman and Caesaria Edmond Benjamin de Rothchild Foundation (V.R.D.). None of the granting agencies participated in any aspect of the study.

Funders
Horowitz Foundation for Applied Biomedical Research
Israel Ministry of Health
Weinkselbaum family medical research fund
Israel Cancer Association

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Chemotherapy testing
Chick embryo
Xenografts

Access to Document

10.1016/j.leukres.2009.02.009

Cite this

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title = "Engraftment of human blood malignancies to the turkey embryo: A robust new in vivo model",

abstract = "Xenografting of human blood malignancies to immunodeficient SCID mice is a powerful research tool. We evaluate here whether the immunodeficient turkey embryo can also serve as a xenograft host for human blood malignancies. Human leukemia, lymphoma and myeloma lines engrafted robustly into medullary and extramedullary tissues of turkey embryos as detected by PCR, FACS and histology in 8-10 days. Four of eleven patient AML samples also engrafted the bone marrow. Grafts of two lines responded to chemotherapy with doxorubicin. The turkey embryo therefore has the potential to be a complementary xenograft model for the study of human blood malignancies.",

keywords = "Chemotherapy testing, Chick embryo, Xenografts",

author = "Igor Grinberg and Arbel Reis and Avivit Ohana and Moran Taizi and Michal Cipok and Sigal Tavor and Deborah Rund and Deutsch, \{Varda R.\} and Goldstein, \{Ronald S.\}",

note = "Funding Information: Thanks to Tsvee Lapidot for providing the G2 cells, to Ada Refaeli for providing the HL-60 line and Galit Granot for the HBL-2 cells. Thanks to Chaya Morgenstern, Dr. Sigi Kay and Shoshana Baron for excellent technical and administrative assistance. Undergraduate interns Alexandra Polsky and Karen Levi provided invaluable assistance. This research was supported by grants by the Horowitz Foundation for Applied Biomedical Research to R.S.G. and the Israel Ministry of Health (Weinkselbaum family medical research fund), the Israel Cancer Association through a donation from Don and Donna Sherman and Caesaria Edmond Benjamin de Rothchild Foundation (V.R.D.). None of the granting agencies participated in any aspect of the study. ",

year = "2009",

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doi = "10.1016/j.leukres.2009.02.009",

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AU - Cipok, Michal

AU - Tavor, Sigal

AU - Rund, Deborah

AU - Deutsch, Varda R.

AU - Goldstein, Ronald S.

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N2 - Xenografting of human blood malignancies to immunodeficient SCID mice is a powerful research tool. We evaluate here whether the immunodeficient turkey embryo can also serve as a xenograft host for human blood malignancies. Human leukemia, lymphoma and myeloma lines engrafted robustly into medullary and extramedullary tissues of turkey embryos as detected by PCR, FACS and histology in 8-10 days. Four of eleven patient AML samples also engrafted the bone marrow. Grafts of two lines responded to chemotherapy with doxorubicin. The turkey embryo therefore has the potential to be a complementary xenograft model for the study of human blood malignancies.

AB - Xenografting of human blood malignancies to immunodeficient SCID mice is a powerful research tool. We evaluate here whether the immunodeficient turkey embryo can also serve as a xenograft host for human blood malignancies. Human leukemia, lymphoma and myeloma lines engrafted robustly into medullary and extramedullary tissues of turkey embryos as detected by PCR, FACS and histology in 8-10 days. Four of eleven patient AML samples also engrafted the bone marrow. Grafts of two lines responded to chemotherapy with doxorubicin. The turkey embryo therefore has the potential to be a complementary xenograft model for the study of human blood malignancies.

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KW - Xenografts

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Engraftment of human blood malignancies to the turkey embryo: A robust new in vivo model

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

Access to Document

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