Abstract
Active bioinspired materials are appealing biotechnological targets, and their study is gaining momentum. These materials, which comprise of an inorganic matrix and one or more biomolecules, are extremely variable and therefore may result difficult to characterize in their intimate structure. In this work we have prepared a hydroxyapatite-l-asparaginase composite, with the perspective of using it in acute leukemia treatment. We demonstrate that the use of electron microscopy and powder X-ray diffraction, combined with the atomic-resolution information coming from solid-state NMR, allows us to understand the topology of the material and how the different components interplay to obtain an active composite.
Original language | English |
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Pages (from-to) | 12719-12726 |
Number of pages | 8 |
Journal | Physical Chemistry Chemical Physics |
Volume | 20 |
Issue number | 18 |
DOIs | |
State | Published - 9 May 2018 |
Bibliographical note
Publisher Copyright:© the Owner Societies 2018.
Funding
This work was supported by Fondazione CR Firenze, MIUR Contract MEDINTECH CTN01_001177_962865, EC Contracts pNMR No. 317127 and iNEXT No. 653706, EU ESFRI Instruct-ERIC (Core Centre CERM Italy), Regione Toscana (CERM-TT, CERTEMA), University of Florence (PIA), FIRC (17941), and IAS ‘‘Hans Fischer Senior fellowship’’ (COFUND). XRD measurements are courtesy of Samuele Ciattini and Laura Chelazzi, CRIST, University of Florence. Elementary analysis is courtesy of Stefania Pucci, Laboratorio di Microanalisi, University of Florence. Shani Hazan (Bar-Ilan University) is acknowledged for help with the analysis and fitting of the 31P CP buildup curves. FEI-Magellan images were collected at the Bar-Ilan University Nanotechnology Center. Gigliola Lusvardi (Universitàdegli Studi di Modena e Reggio Emilia) and Guido Pintacuda (École normale supérieure de Lyon) are acknowledged for fruitful discussions.
Funders | Funder number |
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CERM-TT | |
Horizon 2020 Framework Programme | 653706, 317127 |
International Atherosclerosis Society | |
Ministero dell’Istruzione, dell’Università e della Ricerca | MEDINTECH CTN01_001177_962865 |
Università degli Studi di Firenze | |
Fondazione Italiana per la Ricerca sul Cancro | 17941 |
Regione Toscana | |
Fondazione Cassa di Risparmio di Firenze |