Engineering l-asparaginase for spontaneous formation of calcium phosphate bioinspired microreactors

Alexandra Louka, Irina Matlahov, Stefano Giuntini, Linda Cerofolini, Andrea Cavallo, Serena Pillozzi, Enrico Ravera, Marco Fragai, Annarosa Arcangeli, Ayyalusamy Ramamoorthy, Gil Goobes, Claudio Luchinat

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Active bioinspired materials are appealing biotechnological targets, and their study is gaining momentum. These materials, which comprise of an inorganic matrix and one or more biomolecules, are extremely variable and therefore may result difficult to characterize in their intimate structure. In this work we have prepared a hydroxyapatite-l-asparaginase composite, with the perspective of using it in acute leukemia treatment. We demonstrate that the use of electron microscopy and powder X-ray diffraction, combined with the atomic-resolution information coming from solid-state NMR, allows us to understand the topology of the material and how the different components interplay to obtain an active composite.

Original languageEnglish
Pages (from-to)12719-12726
Number of pages8
JournalPhysical Chemistry Chemical Physics
Volume20
Issue number18
DOIs
StatePublished - 9 May 2018

Bibliographical note

Publisher Copyright:
© the Owner Societies 2018.

Funding

This work was supported by Fondazione CR Firenze, MIUR Contract MEDINTECH CTN01_001177_962865, EC Contracts pNMR No. 317127 and iNEXT No. 653706, EU ESFRI Instruct-ERIC (Core Centre CERM Italy), Regione Toscana (CERM-TT, CERTEMA), University of Florence (PIA), FIRC (17941), and IAS ‘‘Hans Fischer Senior fellowship’’ (COFUND). XRD measurements are courtesy of Samuele Ciattini and Laura Chelazzi, CRIST, University of Florence. Elementary analysis is courtesy of Stefania Pucci, Laboratorio di Microanalisi, University of Florence. Shani Hazan (Bar-Ilan University) is acknowledged for help with the analysis and fitting of the 31P CP buildup curves. FEI-Magellan images were collected at the Bar-Ilan University Nanotechnology Center. Gigliola Lusvardi (Universitàdegli Studi di Modena e Reggio Emilia) and Guido Pintacuda (École normale supérieure de Lyon) are acknowledged for fruitful discussions.

FundersFunder number
CERM-TT
Horizon 2020 Framework Programme653706, 317127
International Atherosclerosis Society
Ministero dell’Istruzione, dell’Università e della RicercaMEDINTECH CTN01_001177_962865
Università degli Studi di Firenze
Fondazione Italiana per la Ricerca sul Cancro17941
Regione Toscana
Fondazione Cassa di Risparmio di Firenze

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