Energy transfer efficiency on lymphocytes of leukemia patients measured by flourescence polarization

Meir Cohen-Kashi; Michal Haran; Elena Afrimzon; Mordechai Deutsch

doi:10.1080/10428190400015121

Energy transfer efficiency on lymphocytes of leukemia patients measured by flourescence polarization

Meir Cohen-Kashi
, Michal Haran
, Elena Afrimzon
, Mordechai Deutsch

Bar-Ilan University
Kaplan Medical Center Israel

Research output: Contribution to journal › Article › peer-review

Abstract

Measurements of fluorescence resonance energy transfer efficiency (E), via fluorescence polarization, have been applied to distinguish between chronic lymphocytic leukemia (CLL) patients and healthy persons. Capping of Concanavalin-A receptors is more pronounced in normal lymphocytes than in those of CLL patients. Membrane capping decreases the distance between donor and acceptor molecules embedded in the membrane (r), and thus increases the monitored energy transfer efficiency (E∼1/r⁶). Blood samples of 10 healthy subjects and 16 CLL patients were examined. In the healthy subjects, the mean E value for capped lymphocytes was 19 ± 3, whereas in the CLL patients it was significantly lower (8 ± 5) (P < 0.01).

Original language	English
Pages (from-to)	113-120
Number of pages	8
Journal	Leukemia and Lymphoma
Volume	46
Issue number	1
DOIs	https://doi.org/10.1080/10428190400015121
State	Published - Jan 2005

Bibliographical note

Funding Information:
The authors wish to thank Prof. Alain Berrebi, head of The Hematology Department, Kaplan Medical Center, for the clinical support of this study. This research was supported by the Horowitz Foundation.

Funding

The authors wish to thank Prof. Alain Berrebi, head of The Hematology Department, Kaplan Medical Center, for the clinical support of this study. This research was supported by the Horowitz Foundation.

Funders
Horowitz Foundation for Social Policy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Capping
Chronic lymphocytic leukemia (CLL)
Fluorescence polarization (FP)
Fluorescence resonance energy transfer (FRET)

Access to Document

10.1080/10428190400015121

Cite this

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title = "Energy transfer efficiency on lymphocytes of leukemia patients measured by flourescence polarization",

abstract = "Measurements of fluorescence resonance energy transfer efficiency (E), via fluorescence polarization, have been applied to distinguish between chronic lymphocytic leukemia (CLL) patients and healthy persons. Capping of Concanavalin-A receptors is more pronounced in normal lymphocytes than in those of CLL patients. Membrane capping decreases the distance between donor and acceptor molecules embedded in the membrane (r), and thus increases the monitored energy transfer efficiency (E∼1/r6). Blood samples of 10 healthy subjects and 16 CLL patients were examined. In the healthy subjects, the mean E value for capped lymphocytes was 19 ± 3, whereas in the CLL patients it was significantly lower (8 ± 5) (P < 0.01).",

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author = "Meir Cohen-Kashi and Michal Haran and Elena Afrimzon and Mordechai Deutsch",

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AU - Cohen-Kashi, Meir

AU - Haran, Michal

AU - Afrimzon, Elena

AU - Deutsch, Mordechai

N1 - Funding Information: The authors wish to thank Prof. Alain Berrebi, head of The Hematology Department, Kaplan Medical Center, for the clinical support of this study. This research was supported by the Horowitz Foundation.

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N2 - Measurements of fluorescence resonance energy transfer efficiency (E), via fluorescence polarization, have been applied to distinguish between chronic lymphocytic leukemia (CLL) patients and healthy persons. Capping of Concanavalin-A receptors is more pronounced in normal lymphocytes than in those of CLL patients. Membrane capping decreases the distance between donor and acceptor molecules embedded in the membrane (r), and thus increases the monitored energy transfer efficiency (E∼1/r6). Blood samples of 10 healthy subjects and 16 CLL patients were examined. In the healthy subjects, the mean E value for capped lymphocytes was 19 ± 3, whereas in the CLL patients it was significantly lower (8 ± 5) (P < 0.01).

AB - Measurements of fluorescence resonance energy transfer efficiency (E), via fluorescence polarization, have been applied to distinguish between chronic lymphocytic leukemia (CLL) patients and healthy persons. Capping of Concanavalin-A receptors is more pronounced in normal lymphocytes than in those of CLL patients. Membrane capping decreases the distance between donor and acceptor molecules embedded in the membrane (r), and thus increases the monitored energy transfer efficiency (E∼1/r6). Blood samples of 10 healthy subjects and 16 CLL patients were examined. In the healthy subjects, the mean E value for capped lymphocytes was 19 ± 3, whereas in the CLL patients it was significantly lower (8 ± 5) (P < 0.01).

KW - Capping

KW - Chronic lymphocytic leukemia (CLL)

KW - Fluorescence polarization (FP)

KW - Fluorescence resonance energy transfer (FRET)

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Energy transfer efficiency on lymphocytes of leukemia patients measured by flourescence polarization

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

Access to Document

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