Abstract
Measurements of fluorescence resonance energy transfer efficiency (E), via fluorescence polarization, have been applied to distinguish between chronic lymphocytic leukemia (CLL) patients and healthy persons. Capping of Concanavalin-A receptors is more pronounced in normal lymphocytes than in those of CLL patients. Membrane capping decreases the distance between donor and acceptor molecules embedded in the membrane (r), and thus increases the monitored energy transfer efficiency (E∼1/r6). Blood samples of 10 healthy subjects and 16 CLL patients were examined. In the healthy subjects, the mean E value for capped lymphocytes was 19 ± 3, whereas in the CLL patients it was significantly lower (8 ± 5) (P < 0.01).
| Original language | English |
|---|---|
| Pages (from-to) | 113-120 |
| Number of pages | 8 |
| Journal | Leukemia and Lymphoma |
| Volume | 46 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2005 |
Bibliographical note
Funding Information:The authors wish to thank Prof. Alain Berrebi, head of The Hematology Department, Kaplan Medical Center, for the clinical support of this study. This research was supported by the Horowitz Foundation.
Funding
The authors wish to thank Prof. Alain Berrebi, head of The Hematology Department, Kaplan Medical Center, for the clinical support of this study. This research was supported by the Horowitz Foundation.
| Funders | Funder number |
|---|---|
| Horowitz Foundation for Social Policy |
Keywords
- Capping
- Chronic lymphocytic leukemia (CLL)
- Fluorescence polarization (FP)
- Fluorescence resonance energy transfer (FRET)
