Endothelial Progenitor Cells for biomaterial applications: Characterization and interaction with mature endothelial cells (HUVEC)

S. Fuchs, R. E. Unger, M. I. Hermanns, K. Peters, C. J. Kirkpatrick

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Characterization and interaction with mature endothelial cells (HUVEC) were analyzed. Mononuclear cells were isolated from buffy coats by Ficoll gradients and cultured in EGM-2 supplemented with VEGF, bFGF, and IGF. At different points cells were fixed in 2% PFA and permeabilized by ice-cold acetone for staining of intracellular antigens. It was found that when EPC cultured alone, only a few cells were able to differentiate.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Pages1730
Number of pages1
StatePublished - 2004
Externally publishedYes
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: 17 May 200421 May 2004

Publication series

NameTransactions - 7th World Biomaterials Congress

Conference

ConferenceTransactions - 7th World Biomaterials Congress
Country/TerritoryAustralia
CitySydney
Period17/05/0421/05/04

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