Endocrinology: The effect of growth hormone on granulosa cell function during in-vitro fertilization

J. S. Younis, Y. Ezra, A. Brzezinnski, T. Fibich, J. G. Schenker, N. Laufer

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2 Scopus citations


The effect of growth hormone addition to human menopausal gonadotrophin (HMG), after pituitary down-regulation, on granulosa cell function, in in-vitro fertilization (IVF) was evaluated. Growth hormone or placebo were added in a prospective, randomized and double-blind manner to an existing IVF stimulation protocol. Forty-two normal ovulatory women (≤38 years old) with mechanical factor infertility and normal male factor were included in the study. Gonadotrophin-releasing hormone agonist (GnRHa) was given from day 21 of the previous cycle until human chorionic gonadotrophin (HCG) administration. Follicular stimulation with HMG was started after pituitary down-regulation. Growth hormone 12 IU/day or placebo were administered on alternate days, beginning day 1 until day 7 of HMG treatment. Granulosa cell function was evaluated, in all patients, by follicular fluid levels of ovarian steroids and insulin-like growth factor-I (IGF-I). In 14 patients, chosen arbitrarily granulosa lutein cells were cultured in the presence and absence of additional HCG. Follicular fluid levels of oestradiol, progesterone, testosterone and IGF-I were similar in both growth hormone and placebo groups. Basal and post-HCG levels of oestradiol and progesterone did not differ significantly between the two groups of granulosa lutein cell cultures. We conclude that after pituitary down-regulation, in-vivo administration of growth hormone with HMG in young ovulatory women does not seem to affect granulosa cell function when compared to the administration of HMG alone.

Original languageEnglish
Pages (from-to)1588-1592
Number of pages5
JournalHuman Reproduction
Issue number10
StatePublished - Oct 1993
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgements The authors gratefully acknowledge Bio-Technology General (Rehovot, Israel) for their supply and preparation of the growth hormone and placebo vials used in the study and for the performance of IGF-I assays in the serum and follicular fluid. Supported by a grant from the Joint Research Fund of the Hebrew University and Hadassah Hospital Jerusalem, Israel.


  • Granulosa cell
  • Growth hormone
  • In-vitro fertilization


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