TY - JOUR
T1 - Emergence of antigenic variants with in serotype A foot and mouth disease virus in India and evaluation of a new vaccine candidate panel
AU - Rudreshappa, Ajjaiah G.
AU - Sanyal, Aniket
AU - Mohapatra, Jajati K.
AU - Subramaniam, Saravanan
AU - De, Ankan
AU - Das, Biswajit
AU - Singanallur, Nagendrakumar B.
AU - Jangam, Anil K.
AU - Muthukrishnan, Madhanmohan
AU - Villuppanoor, Srinivasan A.
AU - Pattnaik, Bramhadev
PY - 2012/8/17
Y1 - 2012/8/17
N2 - Emergence of genetically and antigenically divergent lineages/genotypes and poor intergenotypic antigenic coverage is a major concern in serotype A foot-and-mouth-disease virus (FMDV) in India. In 2009, to cover antigenic diversity emerged in serotype A virus field isolates, IND40/2000 was selected as the new vaccine strain for incorporation in the trivalent FMD vaccine formulation used in India. Although current vaccine strain (IND40/2000) covers most isolates antigenically, a few VP359-deletion group isolates showed low r-value in routine vaccine matching exercise. The VP359-deletion group within genotype 18 emerged first in late part of 2002 and in 2007 causing outbreaks along with non-deletion isolates of the same genotype. In case of emergence or re-emergence of more antigenically divergent isolates in future, a need for a new vaccine candidate to cover maximum isolates of both deletion and non-deletion group may arise. Four alternate candidate vaccine strains (IND281/2003, IND195/2007, IND360/2007 and IND123/2008) were selected based on set criteria and antigenic relationships with field isolates sampled between 2002 and 2009 were analyzed using a micro-neutralization test. Phylogenetic analysis based on capsid region of serotype A isolates revealed existence of two broad distinct clusters (VP359-deletion and non-deletion group) within genotype 18. The VP359-deletion group has diversified genetically with time giving rise to three different sub-lineages (clade18a, 18b and 18c). The present study indicates that the virus candidates IND281/2003 (VP359-deletion group) and IND195/2007 (non-deletion group) can be used as an adjunct or alternative strain to currently used vaccine strain IND40/2000 in case of emergence of more antigenically divergent isolates in future.
AB - Emergence of genetically and antigenically divergent lineages/genotypes and poor intergenotypic antigenic coverage is a major concern in serotype A foot-and-mouth-disease virus (FMDV) in India. In 2009, to cover antigenic diversity emerged in serotype A virus field isolates, IND40/2000 was selected as the new vaccine strain for incorporation in the trivalent FMD vaccine formulation used in India. Although current vaccine strain (IND40/2000) covers most isolates antigenically, a few VP359-deletion group isolates showed low r-value in routine vaccine matching exercise. The VP359-deletion group within genotype 18 emerged first in late part of 2002 and in 2007 causing outbreaks along with non-deletion isolates of the same genotype. In case of emergence or re-emergence of more antigenically divergent isolates in future, a need for a new vaccine candidate to cover maximum isolates of both deletion and non-deletion group may arise. Four alternate candidate vaccine strains (IND281/2003, IND195/2007, IND360/2007 and IND123/2008) were selected based on set criteria and antigenic relationships with field isolates sampled between 2002 and 2009 were analyzed using a micro-neutralization test. Phylogenetic analysis based on capsid region of serotype A isolates revealed existence of two broad distinct clusters (VP359-deletion and non-deletion group) within genotype 18. The VP359-deletion group has diversified genetically with time giving rise to three different sub-lineages (clade18a, 18b and 18c). The present study indicates that the virus candidates IND281/2003 (VP359-deletion group) and IND195/2007 (non-deletion group) can be used as an adjunct or alternative strain to currently used vaccine strain IND40/2000 in case of emergence of more antigenically divergent isolates in future.
KW - FMDV serotype A
KW - Genotype 18
KW - VP3-deletion group
KW - Vaccine strain selection
UR - http://www.scopus.com/inward/record.url?scp=84862580542&partnerID=8YFLogxK
U2 - 10.1016/j.vetmic.2012.02.035
DO - 10.1016/j.vetmic.2012.02.035
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 22445197
AN - SCOPUS:84862580542
SN - 0378-1135
VL - 158
SP - 405
EP - 409
JO - Veterinary Microbiology
JF - Veterinary Microbiology
IS - 3-4
ER -